Tumor necrosis factor-α–308 genotypes influence inflammatory activity and TNF-α serum concentrations in children with juvenile idiopathic arthritis
| Autor: | Queiroz Mv, Patrícia Pinto, Joao Gomes Pedro, Paula Costa, José Costa, Paulo Nicola, Artur Sousa, Joana Caetano-Lopes, João Eurico Fonseca, José António Melo Gomes, Ana Filipa Mourão, Helena Canhão, Jaime Branco, Maria José Santos, José Teles, João Cavaleiro, Iva Brito |
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| Přispěvatelé: | Repositório da Universidade de Lisboa |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Adolescent Genotype Immunology Arthritis Polymorphism Single Nucleotide Severity of Illness Index Psoriatic arthritis Rheumatology Internal medicine Tumor necrosis factor-α promoter polymorphisms medicine Humans Immunology and Allergy Genetic Predisposition to Disease Child Promoter Regions Genetic Portugal medicine.diagnostic_test Tumor Necrosis Factor-alpha business.industry Juvenile idiopathic arthritis medicine.disease Prognosis Arthritis Juvenile Pathophysiology −308 genotypes Erythrocyte sedimentation rate Female Tumor necrosis factor alpha business Juvenile rheumatoid arthritis |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | The Journal of Rheumatology Copyright © 2009. All rights reserved. OBJECTIVE: Considering the relevance of tumor necrosis factor-α (TNF-α) in the pathophysiology of juvenile idiopathic arthritis (JIA), it is likely that polymorphisms in its promoter area may be relevant in disease susceptibility and activity. We investigated if clinical measures of JIA activity and TNF-α serum concentrations were associated with TNF-α –308 genotypes. METHODS: Portuguese patients with JIA in 5 pediatric rheumatology centers were recruited consecutively, along with a control group of healthy subjects. Demographic and clinical data and blood samples were collected from each patient. DNA was extracted for analysis of TNF-α gene promoter polymorphisms at position –308 by restriction fragment-length polymorphism. RESULTS: One hundred fourteen patients and 117 controls were evaluated; 57% of patients presented the oligoarticular subtype, 25% the polyarticular subtype, 8% the systemic subtype, and 9% had enthesitis-related arthritis and 5% psoriatic arthritis. Twenty-four percent of the patients presented the –308 GA/AA genotypes and 76% the –308 GG genotype, similar to findings in controls. Patients with the –308 GA/AA genotype had higher degree of functional impairment, erythrocyte sedimentation rate, 100-mm visual analog scale score for disease activity, and TNF-α levels compared to those with the –308 GG genotype. CONCLUSION: TNF-α –308 GA/AA genotypes were found to be related to higher inflammatory activity and worse measures of disease activity in Portuguese patients with JIA. They were not associated with susceptibility to JIA. Supported by a research grant from AstraZeneca and Faculdade de Medicina da Universidade de Lisboa. |
| Databáze: | OpenAIRE |
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