Insights into centriole geometry revealed by cryotomography of doublet and triplet centrioles
| Autor: | Bettina Keszthelyi, David A. Agard, Ronald D. Vale, Garrett Greenan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Electron Microscope Tomography Centriole QH301-705.5 1.1 Normal biological development and functioning Science Geometry CHO Cells Microtubules General Biochemistry Genetics and Molecular Biology Olfactory Receptor Neurons 03 medical and health sciences Cricetulus Ciliary axoneme Microtubule Underpinning research cell biology Animals centriole Singlet state Cilia Biology (General) Centrioles General Immunology and Microbiology D. melanogaster Chemistry General Neuroscience Cilium Cryoelectron Microscopy General Medicine Cell Biology cryo-electron tomography 030104 developmental biology Drosophila melanogaster centrosome Centrosome Cryo-electron tomography Medicine Generic health relevance Biochemistry and Cell Biology Other Research Article microtubule |
| Zdroj: | eLife eLife, Vol 7 (2018) |
| ISSN: | 2050-084X |
| Popis: | Centrioles are cylindrical assemblies comprised of 9 singlet, doublet, or triplet microtubules, essential for the formation of motile and sensory cilia. While the structure of the cilium is being defined at increasing resolution, centriolar structure remains poorly understood. Here, we used electron cryo-tomography to determine the structure of mammalian (triplet) and Drosophila (doublet) centrioles. Mammalian centrioles have two distinct domains: a 200 nm proximal core region connected by A-C linkers, and a distal domain where the C-tubule is incomplete and a pair of novel linkages stabilize the assembly producing a geometry more closely resembling the ciliary axoneme. Drosophila centrioles resemble the mammalian core, but with their doublet microtubules linked through the A tubules. The commonality of core-region length, and the abrupt transition in mammalian centrioles, suggests a conserved length-setting mechanism. The unexpected linker diversity suggests how unique centriolar architectures arise in different tissues and organisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|