CIA2 and CIA2‐LIKE are required for optimal photosynthesis and stress responses in Arabidopsis thaliana

Autor: Jakub Mielecki, Magdalena Zaborowska, Lars B. Scharff, Dario Leister, Piotr Gawroński, Magdalena Górecka, Stanislaw Karpinski, Paweł Burdiak, Cezary Waszczak
Přispěvatelé: Organismal and Evolutionary Biology Research Programme, Plant ROS-Signalling, Viikki Plant Science Centre (ViPS)
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Chloroplasts
Arabidopsis thaliana
Arabidopsis
Plant Science
thermo‐
01 natural sciences
Chloroplast Proteins
chloroplast translation
Gene Expression Regulation
Plant
CIL
TRANSCRIPTION FACTOR
GENE-EXPRESSION
RIBOSOMAL-PROTEINS
SINGLET OXYGEN
biology
food and beverages
non‐
photochemical quenching
Plants
Genetically Modified
Cell biology
Chloroplast
DNA-BINDING PROTEIN
Signal Transduction
ULTRAVIOLET-RADIATION
Nuclear gene
ENCODED PROTEINS
ASCORBATE PEROXIDASE
03 medical and health sciences
Stress
Physiological
Genetics
Plastid
Cell Nucleus
SIMULATING DISEASE 1
photosynthesis
chloroplast retrograde signaling
Arabidopsis Proteins
Cell Biology
11831 Plant biology
biology.organism_classification
ORGANELLE BIOGENESIS
and photooxidative stress tolerance
CIA2
030104 developmental biology
Diuron
Retrograde signaling
Organelle biogenesis
Heat-Shock Response
Transcription Factors
010606 plant biology & botany
Zdroj: Gawroński, P, Burdiak, P, Scharff, L B, Mielecki, J, Górecka, M, Zaborowska, M, Leister, D, Waszczak, C & Karpiński, S 2021, ' CIA2 and CIA2-LIKE are required for optimal photosynthesis and stress responses in Arabidopsis thaliana ', Plant Journal, vol. 105, no. 3, pp. 619-638 . https://doi.org/10.1111/tpj.15058
ISSN: 1365-313X
0960-7412
Popis: Chloroplast-to-nucleus retrograde signaling is essential for cell function, acclimation to fluctuating environmental conditions, plant growth and development. The vast majority of chloroplast proteins are nuclear-encoded, and must be imported into the organelle after synthesis in the cytoplasm. This import is essential for the development of fully functional chloroplasts. On the other hand, functional chloroplasts act as sensors of environmental changes and can trigger acclimatory responses that influence nuclear gene expression. Signaling via mobile transcription factors (TFs) has been recently recognized as a way of communication between organelles and the nucleus. In this study, we performed a targeted reverse genetic screen to identify dual-localized TFs involved in chloroplast retrograde signaling during stress responses. We found that CHLOROPLAST IMPORT APPARATUS 2 (CIA2) has a functional plastid transit peptide, and can be located both in chloroplasts and the nucleus. Further, we found that CIA2, along with its homolog CIA2-like (CIL) are involved in the regulation of Arabidopsis responses to UV-AB, high light and heat shock. Finally, our results suggest that both CIA2 and CIL are crucial for chloroplast translation. Our results contribute to a deeper understanding of signaling events in the chloroplast-nucleus cross-talk.
Databáze: OpenAIRE
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