The effect of heat- and auto-polymerized denture base polymers on clonogenicity, apoptosis, and necrosis in fibroblasts: denture base polymers induce apoptosis and necrosis
| Autor: | Bjørn Tore Gjertsen, Stein Atle Lie, Berit B. Tysnes, Mihaela Roxana Cimpan, Roald Matre, Nils Skaug, Lill Irene Cressey |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Programmed cell death
Denture Bases Necrosis Cell Apoptosis Biocompatible Materials Flow cytometry Colony-Forming Units Assay Mice L Cells Materials Testing medicine Animals Polymethyl Methacrylate Annexin A5 Clonogenic assay General Dentistry medicine.diagnostic_test Chemistry G1 Phase Biomaterial General Medicine medicine.anatomical_structure Immunology Biophysics medicine.symptom Cell Division |
| Zdroj: | Acta odontologica Scandinavica. 58(5) |
| ISSN: | 0001-6357 |
| Popis: | Eluates from poly(methyl methacrylate)-based denture base polymers have recently been found to enhance death by apoptosis and necrosis in U-937 human monoblastoid cells. The present study investigated the potential of such polymers to induce apoptosis and/or necrosis and to alter clonogenicity in L929 murine fibroblasts. A fibroblast cell line was chosen because the impairment of fibroblasts subjacent to denture bases may result in a weaker or more permeable mucosa. Two aspects were addressed: the effect of direct contact with the denture base polymers and the effect of eluates extracted from the polymers. For this purpose L929 fibroblasts were seeded on disks manufactured from three heat-polymerized and four autopolymerized denture base polymers or in different concentrations of their eluates. The effects were evaluated by light, fluorescent, confocal and electron microscopy, counting of colonies, and flow cytometry. Disks and eluates of all polymers enhanced cell death by apoptosis and necrosis in L929 cells and decreased their clonogenic potential in a dose-dependent manner. Apoptosis was the main form of cell death. In general, the deleterious effects were stronger when cells were plated directly on the polymer disks than in the eluates. The autopolymerized polymers, except one, yielded higher percentages of apoptosis and necrosis than the heat-polymerized polymers. The results of the study indicated that poly(methyl methacrylate)-based denture base polymers trigger death-signals in L929 fibroblasts and open doors for possible modulation of the cell/biomaterial interaction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|