Effective blotting of ultrathin polyacrylamide gels anchored to a solid matrix
| Autor: | Jochen Heukeshoven, Rudolf Dernick |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Chromatography
Materials science Isoelectric focusing Diffusion Blotting Western Clinical Biochemistry Polyacrylamide Acrylic Resins Proteins Sodium Dodecyl Sulfate Biochemistry Buffer (optical fiber) Analytical Chemistry Matrix (chemical analysis) Blot Electrophoresis chemistry.chemical_compound chemistry Electrophoresis Polyacrylamide Gel Isoelectric Focusing Sodium dodecyl sulfate |
| Zdroj: | Electrophoresis. 16:748-756 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.11501601123 |
| Popis: | Ultrathin polyacrylamide gels bound on glass plates or plastic sheets cannot be removed from their support without destruction. Therefore electrophoretic transfer methods are not applicable. We have developed a fast diffusion blotting procedure which is very simple and does not need any equipment like blotting chamber or power supply. Furthermore, no special buffer solutions are required. The method is universally applicable to ultrathin sodium dodecyl sulfate, native as well as isoelectric focusing polyacrylamide gels1 . |
| Databáze: | OpenAIRE |
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