Sour cherry (Prunus cerasus) seed extract increases heme oxygenase-1 expression and decreases proinflammatory signaling in peripheral blood human leukocytes from rheumatoid arthritis patients
| Autor: | Fadia Mahmoud, Adel Al-Awadhi, Ali A. Dashti, Rana Al-Awadhi, Bashayer Al-Zayer, Bela Juhasz, Basel Ibrahim, Arpad Tosaki, David D. Haines |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Lipopolysaccharides Chemokine Lipopolysaccharide CD3 Complex T cell T-Lymphocytes Immunology medicine.disease_cause Proinflammatory cytokine Arthritis Rheumatoid chemistry.chemical_compound Immunology and Allergy Medicine Humans Interleukin 8 Cells Cultured Pharmacology biology business.industry Plant Extracts Tumor Necrosis Factor-alpha Interleukin-8 Middle Aged Heme oxygenase medicine.anatomical_structure chemistry Seeds biology.protein Leukocytes Mononuclear Tumor necrosis factor alpha Female Prunus business Oxidative stress Heme Oxygenase-1 |
| Zdroj: | International immunopharmacology. 20(1) |
| ISSN: | 1878-1705 |
| Popis: | Sour cherry seed extract (SCE) was evaluated for its capacity to inhibit lipopolysaccharide-treated human peripheral blood T cells expressing tumor necrosis factor-alpha, and the chemokine interleukin-8. Both proteins are diagnostic biomarkers for inflammatory pathologies. Peripheral blood leukocytes from 11 rheumatoid arthritis (RA) patients and 8 healthy control subjects were co-cultured for 24 h in lipopolysaccharide and the extract, then evaluated by flow cytometry for T cell activation and by enzyme-linked immunoassay for lymphocyte-associated heme oxygenase-1 (HO-1) expression. There was a dose-dependent decrease in expression of the immunophenotypes: CD3+TNF-α +, and CD3+IL8 + in cultures from RA patients to a greater extent than in cells from healthy participants. These results suggest that the extract may have a modulatory roll in RA and other inflammatory disorders via the induction of HO-1, thus abating oxidative stress and strengthening regulation of pro-inflammatory signaling pathways. |
| Databáze: | OpenAIRE |
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