Influence of the charge of low molecular weight proteins on their efficacy of filtration and/or adsorption on dialysis membranes with different intrinsic properties
| Autor: | M. Thomas, C. Boullanger, S. Fraud, G. Quash, E. Vial, N. Moachon |
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| Rok vydání: | 2002 |
| Předmět: |
Biophysics
Bioengineering Biocompatible Materials law.invention Biomaterials chemistry.chemical_compound Structure-Activity Relationship Adsorption law Renal Dialysis Humans Filtration Creatinine Chromatography Chemistry Beta-2 microglobulin Proteins Membranes Artificial Amyloidosis Blood proteins Molecular Weight Kinetics Membrane Mechanics of Materials Ceramics and Composites Urea Muramidase Lysozyme beta 2-Microglobulin Dialysis |
| Zdroj: | Biomaterials. 23(3) |
| ISSN: | 0142-9612 |
| Popis: | Hemodialysis membranes eliminate by filtration low-molecular-weight toxic metabolites (urea and creatinine) with minimum interactions between blood components and the membrane itself. However, the ability of a membrane to adsorb specific proteins could be beneficial if the accumulation of these same proteins is implicated in the genesis of a pathological condition. β -amyloidosis which accompanies the elevation of β 2 -microglobulin (11.8 kDa) in the plasma of dialysed patients is one such condition (Biochem. Biophys. Res. Commun. 129 (3) (1985) 701–706; Lancet 1 (1986) 1240–1311). To determine whether increases in plasma β 2 -microglobulin levels were due to differences in filtration efficacy of the membrane used and/or to certain characteristics of this protein, e.g. its charge (pI 5.7) the adsorption and filtration of [ 3 H] β 2 -microglobulin and [ 3 H] lysozyme of similar MW 14.5 kDa, but pI: 10.8 were compared on different membranes. It was found that, neither [ 3 H] β 2 -microglobulin nor [ 3 H] lysozyme are removed by cuprophan, whereas over 75% of β 2 -microglobulin is removed by filtration on polyacrylonitrile, polyacrylonitrile–polyethyleneimine, polysulfone and >95% by adsorption to polymethylmethacrylate–BK. For lysozyme, removal by adsorption is >95% on polyacrylonitrile and polyacrylonitrile–polyethyleneimine, 72% on polymethylmethacrylate–BK and by filtration is 95% on polysulfone. Hemodialysis membranes must therefore not simply be considered as filters of low-molecular-weight metabolites but should be equally assessed for their capacity to eliminate potentially deleterious low-molecular-weight plasma proteins. |
| Databáze: | OpenAIRE |
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