Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis
Autor: | Debora Decote-Ricardo, Denise Cristina de Souza Matos, Geraldo R. G. Armôa, Lanuza A P Faccioli, Paula M. De Luca, Suzana Corte-Real, Sergio C.F. Mendonça, Elezer Monte Blanco Lemes, Léa Cysne-Finkelstein |
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Rok vydání: | 2010 |
Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Blotting Western Leishmania mexicana Cell lcsh:QR1-502 Protozoan Proteins lcsh:Microbiology Microbiology Flow cytometry Mice metacyclogenesis expression parasitic diseases medicine Animals KMP-11 Amastigote Mice Inbred BALB C biology medicine.diagnostic_test Immunochemistry Membrane Proteins Leishmaniasis Flow Cytometry biology.organism_classification medicine.disease Leishmania amastigote promastigote Cell biology Microscopy Electron medicine.anatomical_structure Membrane protein Protozoa Female Intracellular Leishmania amazonensis |
Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 105, Iss 3, Pp 341-347 (2010) Memórias do Instituto Oswaldo Cruz, Volume: 105, Issue: 3, Pages: 341-347, Published: MAY 2010 |
ISSN: | 0074-0276 |
DOI: | 10.1590/s0074-02762010000300018 |
Popis: | Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid protozoa, is considered a potential candidate for a leishmaniasis vaccine. A suitable leishmaniasis vaccine candidate molecule must be expressed in amastigotes, the infective stage for mammals. However, the expression of KMP-11 in Leishmania amastigotes has been a subject of controversy. We evaluated the expression of this molecule in logarithmic and stationary growth phase promastigotes, as well as in amastigotes, of Leishmania amazonensis by immunoblotting, flow cytometry and immunocytochemistry, using a monoclonal antibody against KMP-11. We found that KMP-11 is present in promastigotes and amastigotes. In both stages, the protein was found in association with membrane structures (at the cell surface, flagellar pocket and intracellular vesicles). More importantly, its surface expression is higher in amastigotes than in promastigotes and increases during metacyclogenesis. The increased expression of KMP-11 in metacyclic promastigotes, and especially in amastigotes, indicates a role for this molecule in the parasite relationship with the mammalian host. The presence of this molecule in amastigotes is consistent with the previously demonstrated immunoprotective capacity of vaccine prototypes based on the KMP-11-coding gene and the presence of humoral and cellular immune responses to KMP-11 in Leishmania-infected humans and animals. |
Databáze: | OpenAIRE |
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