Sitagliptin prevents the development of metabolic and hormonal disturbances, increased β-cell apoptosis and liver steatosis induced by a fructose-rich diet in normal rats

Autor: Viviana Graciela Madrid, Bárbara Maiztegui, Oscar R. Rebolledo, M. A. Raschia, Flavio Francini, Hector Herminio del Zotto, Luis Emilio Flores, María Laura Massa, Juan José Gagliardino, M I Borelli
Rok vydání: 2010
Předmět:
Blood Glucose
Male
medicine.medical_treatment
Drug Evaluation
Preclinical
Apoptosis
Impaired glucose tolerance
Eating
Insulin-Secreting Cells
Metabolic Syndrome
Fatty liver
Metabolic disorder
Otras Medicina Básica
General Medicine
Medicina Básica
Sitagliptin
Pyrazines
hormones
hormone substitutes
and hormone antagonists
INSULIN RESISTANCE
medicine.drug
medicine.medical_specialty
SITAGLIPTIN
CIENCIAS MÉDICAS Y DE LA SALUD
Drinking
Fructose
Biology
Sitagliptin Phosphate
Insulin resistance
Internal medicine
medicine
PRE-DIABETES
Animals
Hypoglycemic Agents
Rats
Wistar
EXENDIN-4
Dipeptidyl-Peptidase IV Inhibitors
Venoms
Insulin
Body Weight
β-CELL MASS
nutritional and metabolic diseases
Glucose Tolerance Test
Triazoles
medicine.disease
Diet
Rats
Fatty Liver
Endocrinology
HYPERTRIGLYCERIDAEMIA
Exenatide
Steatosis
Peptides
Zdroj: Clinical science (London, England : 1979). 120(2)
ISSN: 1470-8736
Popis: The aim of the present study was to test the effect of sitagliptin and exendin-4 upon metabolic alterations, β-cell mass decrease and hepatic steatosis induced by F (fructose) in rats. Normal adult male Wistar rats received a standard commercial diet without (C) or with 10% (w/v) F in the drinking water (F) for 3 weeks; animals from each group were randomly divided into three subgroups: untreated (C and F) and simultaneously receiving either sitagliptin (CS and FS; 115.2 mg/day per rat) or exendin-4 (CE and FE; 0.35 nmol/kg of body weight, intraperitoneally). Water and food intake, oral glucose tolerance, plasma glucose, triacylglycerol (triglyceride), insulin and fructosamine concentration, HOMA-IR [HOMA (homoeostasis model assessment) for insulin resistance], HOMA-β (HOMA for β-cell function) and liver triacylglycerol content were measured. Pancreas immunomorphometric analyses were also performed. IGT (impaired glucose tolerance), plasma triacylglycerol, fructosamine and insulin levels, HOMA-IR and HOMA-β indexes, and liver triacylglycerol content were significantly higher in F rats. Islet β-cell mass was significantly lower in these rats, due to an increase in the percentage of apoptosis. The administration of exendin-4 and sitagliptin to F animals prevented the development of all the metabolic disturbances and the changes in β-cell mass and fatty liver. Thus these compounds, useful in treating Type 2 diabetes, would also prevent/delay the progression of early metabolic and tissue markers of this disease. Fil: Maiztegui, Barbara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina Fil: Borelli, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina Fil: Madrid, Viviana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina Fil: del Zotto, Hector Herminio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina Fil: Raschia, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina Fil: Francini, Flavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina Fil: Massa, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina Fil: Flores, Luis Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina Fil: Rebolledo, Oscar R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Endocrinología Experimental y Aplicada (i); Argentina
Databáze: OpenAIRE
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