Isotype specific ELISAs to detect antibodies against swine vesicular disease virus and their use in epidemiology
| Autor: | C. Terpstra, F. Van Hemert-Kluitenberg, C. Baars, Aldo Dekker |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Time Factors
Epidemiology medicine.drug_class Swine monoclonal-antibodies Enzyme-Linked Immunosorbent Assay medicine.disease_cause Monoclonal antibody Antibodies Viral Virus CIDC - Division Virology medicine Enterovirus Infections Animals Swine vesicular disease biology CIDC - Divisie Virologie pigs Isotype Virology infection Enterovirus B Human Swine Vesicular Disease Virus Epidemiologic Studies Infectious Diseases Immunoglobulin M Immunology Antibody Formation responses biology.protein Enterovirus Viral disease Antibody Research Article |
| Zdroj: | Epidemiology and Infection, 128(2), 277-284 Epidemiology and Infection 128 (2002) 2 |
| ISSN: | 0950-2688 |
| Popis: | Isotype specific ELISAs to detect antibodies against swine vesicular disease, which may help to estimate the moment of infection, were developed and validated on sera from pigs experimentally infected with four different isolates of swine vesicular disease virus. Virus specific IgM antibodies could be detected from days 3–49 and occasionally up to day 91 after infection. IgG1 antibodies were first detected at day 8 and IgG2 at day 11. IgA antibodies coincided with IgG1 antibodies, but antibody titres varied widely. From the results obtained with the sera from the experimentally infected pigs, we calculated the day at which 50% of the pigs had become positive (D50). A D50 of 5, 4, 12, 12 and 24 days was calculated, respectively, for the appearance of antibodies in the virus neutralization test, the IgM, total IgG, IgG1 and IgG2 ELISA. A D50 of 49 days was calculated for the disappearance of IgM antibodies. The isotype specific ELISAs proved to be valuable tools to study the epidemiology of the disease. |
| Databáze: | OpenAIRE |
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