Exposure of Human Gastric Cells to Oxidized Lipids Stimulates Pathways of Amino Acid Biosynthesis on a Genomic and Metabolomic Level

Autor:	Mathias Zaunschirm, Mark M. Somoza, Marc Pignitter, Nicole Kretschy, Antonio Kopic, Veronika Somoza, Claudia Keßler, Christina Maria Hochkogler
Rok vydání:	2019
Předmět:	Linoleic acid Pharmaceutical Science 01 natural sciences Hexanal Article Analytical Chemistry Linoleic Acid Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Metabolomics linoleic acid peroxidation products Drug Discovery Hexanes Humans Amino Acids Physical and Theoretical Chemistry gastric cells Amino acid synthesis 030304 developmental biology cDNA microarray chemistry.chemical_classification 0303 health sciences 010401 analytical chemistry Organic Chemistry Genomics Compartment (chemistry) metabolomics 3. Good health 0104 chemical sciences hexane chemistry Biochemistry Chemistry (miscellaneous) Molecular Medicine Lipid Peroxidation Oxidation-Reduction Intracellular Polyunsaturated fatty acid
Zdroj:	Molecules Volume 24 Issue 22
ISSN:	1420-3049
DOI:	10.3390/molecules24224111
Popis:	The Western diet is characterized by a high consumption of heat-treated fats and oils. During deep-frying processes, vegetable oils are subjected to high temperatures which result in the formation of lipid peroxidation products. Dietary intake of oxidized vegetable oils has been associated with various biological effects, whereas knowledge about the effects of structurally-characterized lipid peroxidation products and their possible absorption into the body is scarce. This study investigates the impact of linoleic acid, one of the most abundant polyunsaturated fatty acids in vegetable oils, and its primary and secondary peroxidation products, 13-HpODE and hexanal, on genomic and metabolomic pathways in human gastric cells (HGT-1) in culture. The genomic and metabolomic approach was preceded by an up-to-six-hour exposure study applying 100 µ M of each test compound to the apical compartment in order to quantitate the compounds&rsquo recovery at the basolateral side. Exposure of HGT-1 cells to either 100 µ M linoleic acid or 100 µ M 13-HpODE resulted in the formation of approximately 1 µ M of the corresponding hydroxy fatty acid, 13-HODE, in the basolateral compartment, whereas a mean concentration of 0.20 ± 0.13 µ M hexanal was quantitated after an equivalent application of 100 µ M hexanal. An integrated genomic and metabolomic pathway analysis revealed an impact of the linoleic acid peroxidation products, 13-HpODE and hexanal, primarily on pathways related to amino acid biosynthesis (p < 0.05), indicating that peroxidation of linoleic acid plays an important role in the regulation of intracellular amino acid biosynthesis.
Databáze:	OpenAIRE
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