Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray
| Autor: | Mathilde Laird, Fuyuhiko Tamanoi, Hiroyuki Saitoh, Tan Le Hoang Doan, Toshiki Tajima, Kotaro Matsumoto, Yuya Higashi, Ryo Yasuda, Ayumi Shiro, Tetsuya Kawachi, Yue Ma, Albane Birault, Shanmugavel Chinnathambi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Science
Nanoparticle Apoptosis Article 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine Nanoscience and technology Cell Line Tumor Neoplasms Spheroids Cellular Humans DNA Breaks Double-Stranded Irradiation Organic Chemicals Cancer Multidisciplinary TUNEL assay Chemistry X-Rays DNA Breaks Spheroid X-ray Mesoporous silica Silicon Dioxide K-edge 030220 oncology & carcinogenesis Biophysics Medicine Nanoparticles Monochromatic color Porosity Iodine |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| ISSN: | 2045-2322 |
| Popis: | X-ray irradiation of high Z elements causes photoelectric effects that include the release of Auger electrons that can induce localized DNA breaks. We have previously established a tumor spheroid-based assay that used gadolinium containing mesoporous silica nanoparticles and synchrotron-generated monochromatic X-rays. In this work, we focused on iodine and synthesized iodine-containing porous organosilica (IPO) nanoparticles. IPO were loaded onto tumor spheroids and the spheroids were irradiated with 33.2 keV monochromatic X-ray. After incubation in CO₂ incubator, destruction of tumor spheroids was observed which was accompanied by apoptosis induction, as determined by the TUNEL assay. By employing the γH2AX assay, we detected double strand DNA cleavages immediately after the irradiation. These results suggest that IPO first generate double strand DNA breaks upon X-ray irradiation followed by apoptosis induction of cancer cells. Use of three different monochromatic X-rays having energy levels of 33.0, 33.2 and 33.4 keV as well as X-rays with 0.1 keV energy intervals showed that the optimum effect of all three events (spheroid destruction, apoptosis induction and generation of double strand DNA breaks) occurred with a 33.2 keV monochromatic X-ray. These results uncover the preferential effect of K-edge energy X-ray for tumor spheroid destruction mediated by iodine containing nanoparticles. アインシュタインの光電効果をがん細胞の中で再現 放射線治療への新展開. 京都大学プレスリリース. 2021-07-14. Quantum physics helps destroy cancer cells. 京都大学プレスリリース. 2021-07-14. |
| Databáze: | OpenAIRE |
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