Isolation of myogenic progenitor cell population from human placenta: A pilot study
| Autor: | Kyle Herout, Rebecca Stark, Priyadarsini Kumar, Diana L. Farmer, Lee Lankford, Chelsey Lee, Y. Julia Chen, Sandra K Kabagambe, Aijun Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Placenta Population Immunocytochemistry Pilot Projects Biology Muscle Development Flow cytometry 03 medical and health sciences 0302 clinical medicine Pregnancy medicine Humans Progenitor cell education In Situ Hybridization Fluorescence education.field_of_study medicine.diagnostic_test Stem Cells General Medicine Flow Cytometry Molecular biology Immunohistochemistry 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Pregnancy Trimester Second embryonic structures Pediatrics Perinatology and Child Health Immunology Chorionic villi Surgery Female Chorionic Villi Fluorescence in situ hybridization Explant culture |
| Zdroj: | Journal of pediatric surgery. 52(12) |
| ISSN: | 1531-5037 |
| Popis: | Purpose The purpose of this study was to demonstrate a method of isolating myogenic progenitor cells from human placenta chorionic villi and to confirm the myogenic characteristics of the isolated cells. Methods Cells were isolated from chorionic villi of a second trimester male placenta via a combined enzymatic digestion and explant culture. A morphologically distinct subpopulation of elongated and multinucleated cells was identified. This subpopulation was manually passaged from the explant culture, expanded, and analyzed by fluorescence in situ hybridization (FISH) assay, immunocytochemistry, and flow cytometry. Myogenic characteristics including alignment and fusion were tested by growing these cells on aligned polylactic acid microfibrous scaffold in a fusion media composed of 2% horse serum in Dulbecco's modified Eagle medium/high glucose. Results The expanded subpopulation was uniformly positive for integrin α-7. Presence of Y-chromosome by FISH analysis confirmed chorionic villus origin rather than maternal cell contamination. Isolated cells grew, aligned, and fused on the microfibrous scaffold, and they expressed myogenin, desmin, and MHC confirming their myogenic identity. Conclusion Myogenic progenitor cells can be isolated from human chorionic villi. This opens the possibility for translational and clinical applications using autologous myogenic cells for possible engraftment in treatment of chest and abdominal wall defects. |
| Databáze: | OpenAIRE |
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