Evaluation of the Hemocompatibility and Anticancer Potential of Poly(ε-Caprolactone) and Poly(3-Hydroxybutyrate) Microcarriers with Encapsulated Chrysin
| Autor: | Theodore Lialiaris, Anastasia A. Pantazaki, Chrysoula Kokotidou, Efstratios Lialiaris, E. Halevas, Alexandra Moschona, Anna Mitraki, Elda Zaimai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Vinyl alcohol
Chemistry poly(ε-caprolactone) and poly(3-hydroxybutyrate) biodegradable polymers lcsh:RS1-441 Pharmaceutical Science Microcarrier 02 engineering and technology 021001 nanoscience & nanotechnology Dispersant lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound chrysin 0302 clinical medicine anticancer activity Pulmonary surfactant human blood compatibility 030220 oncology & carcinogenesis Emulsion drug micro-encapsulation and delivery Surface charge Chrysin 0210 nano-technology Caprolactone Nuclear chemistry |
| Zdroj: | Pharmaceutics Volume 13 Issue 1 Pharmaceutics, Vol 13, Iss 109, p 109 (2021) |
| ISSN: | 1999-4923 |
| DOI: | 10.3390/pharmaceutics13010109 |
| Popis: | In this work, novel chrysin-loaded poly(&epsilon caprolactone) and poly(3-hydroxybutyrate) microcarriers were synthesized according to a modified oil-in-water single emulsion/solvent evaporation method, utilizing poly(vinyl alcohol) surfactant as stabilizer and dispersing agent for the emulsification, and were evaluated for their physico-chemical and morphological properties, loading capacity and entrapment efficiency and in vitro release of their load. The findings suggest that the novel micro-formulations possess a spherical and relatively wrinkled structure with sizes ranging between 2.4 and 24.7 µ m and a highly negative surface charge with z-potential values between (&minus 18.1)&ndash (&minus 14.1) mV. The entrapment efficiency of chrysin in the poly(&epsilon caprolactone) and poly(3-hydroxybutyrate) microcarriers was estimated to be 58.10% and 43.63%, whereas the loading capacity was found to be 3.79% and 15.85%, respectively. The average release percentage of chrysin was estimated to be 23.10% and 18.01%, respectively. The novel micromaterials were further biologically evaluated for their hemolytic activity through hemocompatibility studies over a range of hematological parameters and cytoxicity against the epithelial human breast cancer cell line MDA-MB 231. The poly(&epsilon caprolactone) and poly(3-hydroxybutyrate) microcarriers reached an IC50 value with an encapsulated chrysin content of 149.19 µ M and 312.18 µ M, respectively, and showed sufficient blood compatibility displaying significantly low (up to 2%) hemolytic percentages at concentrations between 5 and 500 µ g· mL&minus 1. |
| Databáze: | OpenAIRE |
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