Magnetic Resonance Imaging–Guided Microdialysis Cannula Implantation in a Spontaneous High-Grade Glioma Murine Model
| Autor: | Angel M. Carcaboso, Lionel M.L. Chow, Clinton F. Stewart, Stacy L. Throm, Ziwei M. Zhang, Mohamed A. Elmeliegy, Suzanne J. Baker, Fan Wang, Christopher Calabrese |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Microdialysis Central nervous system Brain tumor Mice Nude Pharmaceutical Science Antineoplastic Agents Magnetic Resonance Imaging Interventional Article Magnetic resonance angiography Catheterization Catheters Indwelling Glioma medicine Animals Mice Knockout medicine.diagnostic_test Brain Neoplasms business.industry Magnetic resonance imaging Equipment Design Bregma medicine.disease Cannula Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Anatomic Landmarks Neoplasm Grading Nuclear medicine business Magnetic Resonance Angiography |
| Zdroj: | Journal of Pharmaceutical Sciences. 100:4210-4214 |
| ISSN: | 0022-3549 |
| Popis: | Cerebral microdialysis is used to study anticancer drug penetration in the central nervous system (CNS) and brain tumors in animal models. Genetically engineered murine models (GEMMs) have been recently used to study many aspects of CNS tumors since they represent a more relevant model than orthotopic brain tumor xenograft models. However, it is challenging to implant microdialysis cannula in these animals because T2-weighted magnetic resonance imaging (MRI) does not show the reference point (bregma) traditionally used to obtain stereotactic coordinates. Thus, an alternative reference point that can be visualized on MRI images is needed. In this study, a novel reference point, identified as the intersection between the olfactory bulb/frontal lobe border and the midline between cerebral hemispheres on T2-weighted MRI images, was used to calculate anterior–posterior and medial–lateral coordinates of brain tumors in a GEMM. This point overlies a visible crossover between the rostral rhinal vein and the midline suture on the mouse skull, allowing for the conversion of the MRI coordinates into surgical stereotactic coordinates. Postmortem MRI and histological examination confirmed accurate probe placement. This procedure will facilitate the accurate and precise implantation of microdialysis probes for the study of anticancer drug penetration in brain tumors of GEMMs. © 2011 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 100:4210–4214, 2011 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text