Prevalence and in vitro antifungal susceptibility of cryptic species of the genus Aspergillus isolated in clinical samples
| Autor: | María J. Torres, Maite Ruiz, Javier Aznar, María Reyes Vidal-Acuña |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) Species complex Antifungal Agents Adolescent 030106 microbiology Microbial Sensitivity Tests Aspergillosis Microbiology Young Adult 03 medical and health sciences 0302 clinical medicine Genus Amphotericin B medicine Humans 030212 general & internal medicine Child Aged Aged 80 and over Aspergillus biology Infant Middle Aged medicine.disease biology.organism_classification In vitro Type species Child Preschool Female Echinocandins medicine.drug |
| Zdroj: | Enfermedades Infecciosas y Microbiología Clínica. 37:296-300 |
| ISSN: | 0213-005X |
| DOI: | 10.1016/j.eimc.2018.07.010 |
| Popis: | Introduction The genus Aspergillus contains more than 300 species, which are divided into closely related groups called sections. Molecular studies have revealed numerous cryptic species within different sections of this genus, which have different profiles of antifungal susceptibility and lack diagnostic morphological features. However, there are few studies on the prevalence and in vitro antifungal susceptibility of the cryptic species of this genus. The aim of this study was to investigate the distribution of Aspergillus spp. among clinical samples, and to study their in vitro susceptibility to different antifungal drugs. Method Over a period of 2-years (2014–2015), a total of 379 strains of the genus Aspergillus were isolated. Most of the isolates were classified as respiratory colonizations; no cases of invasive aspergillosis were found. The strains were identified by MALDI-TOF mass spectrometry, and susceptibility testing was performed by the EUCAST reference procedure. Results Twenty species belonging to 8 sections were identified, being A. fumigatus the most prevalent (44.1%). The prevalence of cryptic species was 15.3%, with a clear predominance of A. tubingensis. Among the tested antifungal drugs, amphotericin B was the less active in vitro, followed by triazole drugs and echinocandins. The cryptic species had minimun inhibitory concentrations (MICs) higher than the corresponding type species. Conclusions Accurate identification of the genus Aspergillus at the species level and in vitro antifungal susceptibility testing are necessary because, as it has been shown, some species of this genus may show resistance profiles against available antifungal drugs. |
| Databáze: | OpenAIRE |
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