Patterns of proto-oncogene expression in human glioma cell lines
Autor: | Bengt Westermark, R. V. LaRocca, Mark L. Rosenblum, M. A. Israel |
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Rok vydání: | 1989 |
Předmět: |
Central nervous system
Gene Expression Proto-Oncogene Mas Cellular and Molecular Neuroscience Glioma Glial Fibrillary Acidic Protein Proto-Oncogenes Biopsy Tumor Cells Cultured medicine Humans RNA Messenger RNA Neoplasm Northern blot Messenger RNA Glial fibrillary acidic protein biology Oncogene medicine.diagnostic_test Blotting Northern medicine.disease Molecular biology medicine.anatomical_structure Cell culture biology.protein DNA Probes |
Zdroj: | Journal of Neuroscience Research. 24:97-106 |
ISSN: | 1097-4547 0360-4012 |
DOI: | 10.1002/jnr.490240114 |
Popis: | Previously reported studies have suggested that variations in the pattern of proto-oncogene expression within a specific tumor type may denote an underlying difference in the biology and clinical behavior of those tumors. To more sensitively characterize malignant tumors of the central nervous system, we have used Northern blot hybridization analysis to determine the patterns of expression of seven proto-oncogenes in 20 cell lines established from biopsy specimens of patients with malignant glioma. The following proto-oncogenes are expressed at detectable levels in 30 micrograms of total RNA from most glioma cell lines examined: c-myc (20/20), c-mil/raf-1 (18/18), neu (19/20), c-erbB (19/20), and c-myb (17/20). In contrast, only half of the cell lines expressed detectable c-sis (10/20). In none of the cell lines tested was N-myc (0/20) mRNA detected. Morphologic analysis of these 20 cell lines revealed three different growth patterns: bipolar, epithelial, and pleomorphic-glial. Detectable levels of c-sis mRNA typically occurred with either an epithelial or pleomorphic-glial morphology. The pleomorphic-glial subgroup was also characterized by the expression of glial fibrillary acidic protein. |
Databáze: | OpenAIRE |
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