A specific sequence with a bulged guanosine residue(s) in a stem-bulge-stem structure of Rev-responsive element RNA is required for trans activation by human immunodeficiency virus type 1 Rev
| Autor: | S. M. Gerstberger, Sundararajan Venkatesan, Mayra Chavez, Steven M. Holland |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Gene Expression Regulation
Viral Models Molecular Transcriptional Activation Base pair Immunology Response element Molecular Sequence Data Guanosine RNA-binding protein Biology Regulatory Sequences Nucleic Acid Microbiology chemistry.chemical_compound Virology Consensus Sequence Consensus sequence Humans Protein secondary structure Regulation of gene expression Base Sequence Models Genetic RNA RNA-Binding Proteins rev Gene Products Human Immunodeficiency Virus Molecular biology Gene Products rev chemistry Insect Science Mutation HIV-1 Nucleic Acid Conformation RNA Viral Research Article HeLa Cells |
| Popis: | We demonstrate that both the in vitro RNA binding and in vivo trans activation functions of human immunodeficiency virus type 1 Rev regulatory protein Rev require the presence of a 9-nucleotide 5'-CACUAUGGG-3' RNA motif on its cognate target, the Rev-responsive element RNA. For optimal Rev recognition, this sequence must be presented as a stem-bulge-stem structure and must contain at least two G's, one of which must be unpaired, and include some or all of the CACUAU sequence upstream of the three G's. Distal mutations which result in the base pairing of the G's eliminate the Rev response. The first G is crucial, but changes at the other G's are tolerated if at least one G is unpaired. The secondary structure or the three-dimensional orientation of the B1 and B2 stem-loops of the Rev-responsive element are not relevant as long as the 5'-CACUAUGGG-3' sequence is preserved, with at least one bulged G residue. |
| Databáze: | OpenAIRE |
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