Effect of neonatal isolation on the noradrenergic transduction system in the rat hippocampal slice
| Autor: | Shigeru Morinobu, Kouji Kusaka, Shigeto Yamawaki, Ki-ichiro Kawano |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Adrenergic receptor Hippocampus Adrenergic In Vitro Techniques Biology Hippocampal formation Clonidine Calcium in biology Rats Sprague-Dawley Norepinephrine Phenylephrine Cellular and Molecular Neuroscience Pregnancy Internal medicine medicine Animals Dentate gyrus Isoproterenol Adrenergic beta-Agonists Granule cell Rats Endocrinology medicine.anatomical_structure Animals Newborn Social Isolation Calcium Female Pyramidal cell Adrenergic alpha-Agonists |
| Zdroj: | Synapse. 54:223-232 |
| ISSN: | 1098-2396 0887-4476 |
| Popis: | Numerous studies suggest that early adverse experiences induce neurochemical, morphological, and functional changes in the hippocampus in adolescence and adulthood. The aim of this study was to identify the influence of neonatal isolation (NI) on noradrenaline (NA)-mediated intracellular calcium ([Ca2+]i) mobilization. To measure [Ca2+]i, we used the Ca2+-sensitive dye fura-2 and analysis by fluorescence microscopy. First, we examined the contributions of adrenergic receptor subtypes to the NA-stimulated increase in [Ca2+]i in the granule cell layers of the dentate gyrus (DG) and in the pyramidal cell layers of the CA3 in the hippocampus. Second, we found that the NA-stimulated [Ca2+]i increment was significantly decreased in response to NI in these hippocampal regions. In addition, we examined the influence of environmental enrichment (EE) after weaning on the decrease in the NA-stimulated [Ca2+]i increment induced by NI. The administration of EE reversed the influence of NI on the NA-stimulated [Ca2+]i increment in the CA3 pyramidal cell layer but not in the DG granular cell layer in the hippocampus. These findings suggest that NI and EE after weaning may modulate hippocampal function by altering adrenergic receptor-mediated signal transduction during adolescence. Synapse 54:223–232, 2004. © 2004 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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