Distribution, characterization, and antibiotic resistance of hypervirulent Klebsiella pneumoniae isolates in a Chinese population with asymptomatic bacteriuria
| Autor: | Li, Jun, Li, Yanbing, Tang, Mengli, Xia, Fengjun, Min, Changhang, Hu, Yongmei, Wang, Haichen, Zhang, Jingyi, Zou, Mingxiang |
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| Rok vydání: | 2022 |
| Předmět: |
Adult
Male Microbiology (medical) Bacteriuria Virulence Factors Microbial Sensitivity Tests Moths Antimicrobial susceptibility Microbiology Asian People Drug Resistance Multiple Bacterial Animals Humans Hypervirulent K. pneumoniae Biofilm formation Asymptomatic Infections Phylogeny Research Whole-genome sequencing (WGS) Middle Aged QR1-502 Klebsiella Infections Klebsiella pneumoniae Biofilms Larva Female Asymptomatic bacteriuria Virulence-associated genes |
| Zdroj: | BMC Microbiology, Vol 22, Iss 1, Pp 1-9 (2022) BMC Microbiology |
| ISSN: | 1471-2180 |
| DOI: | 10.1186/s12866-021-02413-w |
| Popis: | Background Asymptomatic bacteriuria (ASB) frequently occurs among all ages and may develop into urinary tract infections (UTIs). Hypervirulent Klebsiella pneumoniae (hvKP) has become a new threat to human health. In our study, we aimed to investigate the epidemiological characteristics of hvKP in population with ASB. Results A total of 61 K. pneumoniae isolates were collected from 7530 urine samples between October and December 2020. The strains were sensitive to most of the antimicrobial agents tested, but a polymyxin resistant strain was found (MIC>16 μg/mL). Three serotypes were detected, including K1 (16.4%, 10/61), K5 (1.6%, 1/61) and K57 (3.2%, 2/61). Four strains (KPNY9, KPNY31, KPNY40, and KPNY42) carried a combination of two or more hypervirulent markers (peg-344, iroB, iucA, prmpA, and prmpA2), and their survival rates after Galleria mellonella infection were lower than those of the other strains (40.0 vs. 70.0%), suggesting that they were hvKP. These hvKP strains with lower biofilm forming ability than classical K. pneumoniae (0.2625 ± 0.0579 vs. 0.6686 ± 0.0661, P = 0.033) were identified as belonging to K2-ST65, K2-ST86, K57-ST592, and K2-ST5559 (a new ST type). KPNY31 (ST5559) shared a close genetic relationship with KPNY42 (ST86) and other ST86 isolates, which have been detected in both nosocomial and community-acquired infections. Conclusions The hvKP with relatively weak biofilm formation was detected in a population with ASB, which was more likely to cause bacteremia and serious consequences. A novel sequence type (ST5559) hvKP derived from ST86 was found. Therefore, hvKP should be monitored in the population with ASB. |
| Databáze: | OpenAIRE |
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