Nanoscale insight into the degradation mechanisms of the cartilage articulating surface preceding OA
| Autor: | Thomas M. Schmid, Rosa M. Espinosa-Marzal, Tooba Shoaib, Catherine Yuh, Markus A. Wimmer |
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| Rok vydání: | 2020 |
| Předmět: |
Cartilage
Articular Friction Biomedical Engineering chemistry.chemical_element 02 engineering and technology Osteoarthritis Matrix (biology) Calcium Microscopy Atomic Force 03 medical and health sciences Cartilage surface Joint disease medicine Animals General Materials Science 030304 developmental biology 0303 health sciences Chemistry Atomic force microscopy Cartilage 021001 nanoscience & nanotechnology medicine.disease Bovine Cartilage medicine.anatomical_structure Biophysics Cattle 0210 nano-technology |
| Zdroj: | Biomaterials science. 8(14) |
| ISSN: | 2047-4849 |
| Popis: | Osteoarthritis (OA) is a degenerative joint disease and a leading cause of disability globally. In OA, the articulating surface of cartilage is compromised by fissures and cracks, and sometimes even worn away completely. Due to its avascular nature, articular cartilage has a poor self-healing ability, and therefore, understanding the mechanisms underlying degradation is key for OA prevention and for optimal design of replacements. In this work, the articulating surface of bovine cartilage was investigated in an environment with enhanced calcium concentration -as often found in cartilage in relation to OA- by combining atomic force microscopy, spectroscopy and an extended surface forces apparatus for the first time. The experimental results reveal that increased calcium concentration irreversibly weakens the cartilage's surface layer, and promotes stiction and high friction. The synergistic effect of calcium on altering the cartilage surface's structural, mechanical and frictional properties is proposed to compromise cartilage integrity at the onset of OA. Furthermore, two mechanisms at the molecular level based on the influence of calcium on lubricin and on the aggregation of the cartilage's matrix, respectively, are identified. The results of this work might not only help prevent OA but also help design better cartilage replacements. |
| Databáze: | OpenAIRE |
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