Multiple Genetic Mutations Related to Insecticide Resistance are Detected in Field Kazakhstani House Flies (Muscidae: Diptera)
| Autor: | Jiang Zhu, Xinghui Qiu, Roman Jashenko, Ruina Qu, Mei Li |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Insecticides Biology 01 natural sciences Insecticide Resistance 03 medical and health sciences Carboxylesterase Houseflies Animals Allele 030304 developmental biology Genetics 0303 health sciences General Veterinary Phylogenetic tree Point mutation fungi Haplotype biology.organism_classification Kazakhstan Organophosphates 010602 entomology Infectious Diseases Insecticide resistance Insect Science Muscidae Mutation Insect Proteins Parasitology Carbamates Musca |
| Zdroj: | Journal of Medical Entomology. 58:2338-2348 |
| ISSN: | 1938-2928 0022-2585 |
| DOI: | 10.1093/jme/tjab110 |
| Popis: | The house fly (Musca domestica Linnaeus) is an important disease vector. Insecticide resistance is an obstacle to effective house fly control. Previous studies have demonstrated that point mutations in acetylcholinesterase (Ace), carboxylesterase (MdαE7) and voltage-sensitive sodium channel (Vssc), and over-expression of CYP6D1v1 confer insecticide resistance in the house fly. However, information about the status and underlying mechanisms of insecticide resistance in Kazakhstani house flies is lacking. In this study, we investigated the occurrence of genetic mutations associated with insecticide resistance in field house flies collected at six different locations in southern Kazakhstan. Four mutations (V260L, G342A/V, and F407Y) in Ace and three mutations (G137D and W251L/S) in MdαE7 were detected with appreciable frequencies. Notably, haplotypes carrying triple-loci mutations in Ace and double mutations in MdαE7 were found in Kazakhstan. The L1014H and L1014F mutations in Vssc, and CYP6D1v1 resistance allele were detected at a low frequency in some of the six investigated house fly populations. Phylogenetic analyses of haplotypes supported multiple origins of resistance mutations in Ace and MdαE7. These observations suggest that house flies in southern Kazakhstan may exhibit significant resistance to organophosphates and carbamates. Regular monitoring of insecticide resistance is recommended to achieve effective house fly control by chemical agents in southern Kazakhstan. |
| Databáze: | OpenAIRE |
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