Analysis of the methylation pattern of six gene promoters in sperm of men with abnormal protamination

Autor: Laszlo Nanassy, Douglas T. Carrell
Rok vydání: 2011
Předmět:
Zdroj: Asian journal of andrology. 13(2)
ISSN: 1745-7262
Popis: It has recently been shown that alteration of the methylation pattern of imprinted genes is associated with different types of male infertility. The objective of our study was to investigate the methylation pattern of selected gene promoters in sperm of patients with abnormal protamine replacement. The promoters of OCT4, SOX2, NANOG, HOXC11, miR-17 and CREM were analyzed using bisulfite sequencing and the percentage of DNA methylation was compared between patients with an abnormal protamine 1/protamine 2 (P1/P2) ratio and normozoospermic controls. No significant quantitative differences were found between groups of patients with either an abnormally high or low P1/P2 ratio compared to normal controls. However, two individual samples from infertile subjects (2/20, 10%) showed an altered methylation pattern for the CREM gene promoter that was not found in control samples. These two samples had a significantly higher (P
Databáze: OpenAIRE