Analysis of the methylation pattern of six gene promoters in sperm of men with abnormal protamination
Autor: | Laszlo Nanassy, Douglas T. Carrell |
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Rok vydání: | 2011 |
Předmět: |
Male
CAMP-Responsive Element Modulator Urology Short Communication Bisulfite sequencing Pilot Projects Male infertility Epigenesis Genetic Andrology Cyclic AMP Response Element Modulator Genomic Imprinting medicine Humans Epigenetics Protamines education Promoter Regions Genetic Infertility Male DNA Primers Homeodomain Proteins education.field_of_study biology Base Sequence SOXB1 Transcription Factors General Medicine Methylation Nanog Homeobox Protein DNA Methylation medicine.disease Protamine Molecular biology Spermatozoa MicroRNAs DNA methylation biology.protein Genomic imprinting Octamer Transcription Factor-3 |
Zdroj: | Asian journal of andrology. 13(2) |
ISSN: | 1745-7262 |
Popis: | It has recently been shown that alteration of the methylation pattern of imprinted genes is associated with different types of male infertility. The objective of our study was to investigate the methylation pattern of selected gene promoters in sperm of patients with abnormal protamine replacement. The promoters of OCT4, SOX2, NANOG, HOXC11, miR-17 and CREM were analyzed using bisulfite sequencing and the percentage of DNA methylation was compared between patients with an abnormal protamine 1/protamine 2 (P1/P2) ratio and normozoospermic controls. No significant quantitative differences were found between groups of patients with either an abnormally high or low P1/P2 ratio compared to normal controls. However, two individual samples from infertile subjects (2/20, 10%) showed an altered methylation pattern for the CREM gene promoter that was not found in control samples. These two samples had a significantly higher (P |
Databáze: | OpenAIRE |
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