Imperfect hybrid layers created by an aggressive one-step self-etch adhesive in primary dentin are amendable to biomimetic remineralization in vitro
| Autor: | Ryan M. Vaughn, Kyoung-Kyu Choi, Jongryul Kim, Roy A. Rockman, Lisha Gu, Dwayne Arola, David H. Pashley, Tara E. Schafer, Franklin R. Tay |
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| Rok vydání: | 2009 |
| Předmět: |
Materials science
Composite number Biomedical Engineering One-Step In Vitro Techniques Article Biomaterials Rhodamine Diffusion chemistry.chemical_compound stomatognathic system Microscopy Electron Transmission Biomimetics Microscopy Dentin medicine Humans Composite material Remineralisation Microscopy Confocal Construction Materials Metals and Alloys Adhesiveness Molar Resins Synthetic medicine.anatomical_structure chemistry Transmission electron microscopy Dentin-Bonding Agents Ceramics and Composites Adhesive |
| Zdroj: | Journal of biomedical materials research. Part A. 93(4) |
| ISSN: | 1552-4965 |
| Popis: | Degradation of hybrid layers created in primary dentin occurs as early as 6 months in vivo. Biomimetic remineralization utilizes “bottom-up” nanotechnology principles for interfibrillar and intrafibrillar remineralization of collagen matrices. This study examined whether imperfect hybrid layers created in primary dentin can be remineralized. Coronal dentin surfaces were prepared from extracted primary molars and bonded using Adper Prompt L-Pop and a composite. One millimeter-thick specimen slabs of the resin-dentin interface were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs to mimic the sequestration and templating functions of dentin noncollagenous proteins. Specimens were retrieved after 1–6 months. Confocal laser scanning microscopy was employed for evaluating the permeability of hybrid layers to Rhodamine B. Transmission electron microscopy was used to examine the status of remineralization within hybrid layers. Remineralization at different locations of the hybrid layers corresponded with quenching of fluorescence within similar locations of those hybrid layers. Remineralization was predominantly intrafibrillar in nature as interfibrillar spaces were filled with adhesive resin. Biomimetic remineralization of imperfect hybrid layers in primary human dentin is a potential means for preserving bond integrity. The success of the current proof-of-concept, laterally-diffusing remineralization protocol warrants development of a clinically-applicable biomimetic remineralization delivery system. |
| Databáze: | OpenAIRE |
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