Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system
| Autor: | Andrew J. Syder, John W. Schoggins, Salman R. Khetani, Charles M. Rice, Margaret R. MacDonald, Christopher T. Jones, Lok Man J. Law, Maria Teresa Catanese, Sangeeta N. Bhatia, Alexander Ploss, Thomas S. Oh |
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| Přispěvatelé: | Harvard University--MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Khetani, Salman R., Bhatia, Sangeeta N. |
| Rok vydání: | 2009 |
| Předmět: |
Hepacivirus
medicine.medical_treatment Hepatitis C virus Cell Population Biomedical Engineering Bioengineering Liver transplantation medicine.disease_cause Applied Microbiology and Biotechnology 03 medical and health sciences 0302 clinical medicine Fluorescent cell Computer Systems Genes Reporter medicine education Gene 030304 developmental biology 0303 health sciences education.field_of_study biology Staining and Labeling biology.organism_classification Image Enhancement Virology 3. Good health Genetically modified organism medicine.anatomical_structure Microscopy Fluorescence 030220 oncology & carcinogenesis Molecular Medicine Biotechnology |
| Zdroj: | PMC |
| ISSN: | 1546-1696 |
| Popis: | Author Manuscript 2010 August 1 Hepatitis C virus (HCV), which infects 2–3% of the world population, is a causative agent of chronic hepatitis and the leading indication for liver transplantation1. The ability to propagate HCV in cell culture (HCVcc) is a relatively recent breakthrough and a key tool in the quest for specific antiviral therapeutics. Monitoring HCV infection in culture generally involves bulk population assays, use of genetically modified viruses and/or terminal processing of potentially precious samples. Here we develop a cell-based fluorescent reporter system that allows sensitive distinction of individual HCV-infected cells in live or fixed samples. We demonstrate use of this technology for several previously intractable applications, including live-cell imaging of viral propagation and host response, as well as visualizing infection of primary hepatocyte cultures. Integration of this reporter with modern image-based analysis methods could open new doors for HCV research. New York (State). Dept. of Health (Empire State Stem Cell Fund Contract C023046) United States. Public Health Service (Grant R01 DK56966) National Institutes of Health (U.S.) (Roadmap for Medical Research Grant 1 R01 DK085713-01) Howard Hughes Medical Institute (Investigator) |
| Databáze: | OpenAIRE |
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