Fluorescence enlightens RND pump activity and the intrabacterial concentration of antibiotics
| Autor: | Jelena Pajovic, Julia Vergalli, Matthieu Réfrégiers, Jean-Marie Pagès, Estelle Dumont, Muriel Masi |
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| Přispěvatelé: | Membranes et cibles thérapeutiques (MCT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Biomédicale des Armées (IRBA)-Aix Marseille Université (AMU), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), European Project: 115525,EC:FP7:SP1-JTI,IMI-JU-06-2012,Translocation(2013), rosa, emmanuelle, Molecular basis of the outer membrane permeability - Translocation - - EC:FP7:SP1-JTI2013-01-01 - 2017-12-31 - 115525 - VALID, Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Biomédicale des Armées (IRBA), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA) |
| Rok vydání: | 2017 |
| Předmět: |
drug efflux pumps
0301 basic medicine Gram-negative bacteria Membrane permeability medicine.drug_class [SDV]Life Sciences [q-bio] 030106 microbiology Antibiotics Population transporters Biology Microbiology Fluorescence 03 medical and health sciences Antibiotic resistance Bacterial Proteins multidrug resistance Gram-Negative Bacteria medicine bacterial imaging education [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology Molecular Biology education.field_of_study Antibiotic Membrane Transport Proteins Biological Transport General Medicine biology.organism_classification Anti-Bacterial Agents [SDV] Life Sciences [q-bio] Multiple drug resistance [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology 030104 developmental biology Multigene Family Biophysics Efflux Bacteria |
| Zdroj: | Research in Microbiology Research in Microbiology, Elsevier, 2018, 169 (7-8), pp.432-441. ⟨10.1016/j.resmic.2017.11.005⟩ Research in Microbiology, 2018, 169 (7-8), pp.432-441. ⟨10.1016/j.resmic.2017.11.005⟩ |
| ISSN: | 1769-7123 0923-2508 |
| DOI: | 10.1016/j.resmic.2017.11.005⟩ |
| Popis: | International audience; To understand the antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation which is defined by influx and efflux. Several methods exist to evaluate the membrane permeability and efflux pump activity but they present some disadvantages and limitations. An optimized spectrofluorimetric method using the intrinsic tryptophan fluorescence as internal standard as well as a complementary microfluorimetric assay following the time-course accumulation in intact individual cells have been developed. Comparing the latter population and single cell approaches can lead to the understanding of the phenotypic heterogeneity within a population. The two methodologies lead to the determination of parameters, concentration, accumulation rates, localization that contribute to emerging concepts (RTC2T, SICAR) with the aim to identify and detail the antibiotic chemotypes that are involved in influx/efflux. |
| Databáze: | OpenAIRE |
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