Mortality of Potato Psyllid (Hemiptera: Triozidae) on Host Clippings Inoculated With Ergot Alkaloids
Autor: | W. Rodney Cooper, Jennifer M. Duringer, Ismael E. Badillo-Vargas, Arash Rashed, Navneet Kaur, Gabriela Esparza-Diaz, David R. Horton |
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Rok vydání: | 2020 |
Předmět: |
Nymph
0106 biological sciences Ergot Alkaloids Clavicipitaceae Bactericera cockerelli Ecology biology Ipomoea leptophylla General Medicine biology.organism_classification 010603 evolutionary biology 01 natural sciences Plant use of endophytic fungi in defense Hemiptera Horticulture Ipomoea alba Ipomoea pandurata Ipomoea tricolor Insect Science Hypocreales Animals Convolvulaceae Solanum tuberosum 010606 plant biology & botany |
Zdroj: | Journal of Economic Entomology. 113:2079-2085 |
ISSN: | 1938-291X 0022-0493 |
DOI: | 10.1093/jee/toaa144 |
Popis: | Our previous study provided correlative evidence that morning glory species harboring endophytic fungi (Periglandula) are resistant to potato psyllid [Bactericera cockerelli (Šulc)], whereas species free of fungi often allowed psyllid development. In this study, we manipulated levels of ergot alkaloids in host tissues by inoculating clippings from potato plants with extracts from morning glories that harbor Periglandula [Ipomoea leptophylla Torrey, Ipomoea imperati (Vahl) Grisebach, Ipomoea tricolor Cavanilles, Ipomoea pandurata (L.) G. F. Meyer, and Turbina corymbosa (L.)] and one species (Ipomoea alba L.) that does not harbor the endophyte. Ergot alkaloids (clavines, lysergic acid amides, and ergopeptines) were detected in potato clippings, thus confirming that leaves had taken up compounds from solutions of crude extracts. Psyllid mortality rates on inoculated clippings ranged between 53 and 93% in treatments producing biochemically detectable levels of alkaloids, when compared with 15% mortality in water controls or the alkaloid-free I. alba. We then tested synthetic analogs from each of the three alkaloid classes that had been detected in the crude extracts. Each compound was assayed by inoculating clippings of two host species (potato and tomato) at increasing concentrations (0, 1, 10, and 100 µg/ml in solution). Psyllids exhibited a large and significant increase in mortality rate beginning at the lowest two concentrations, indicating that even very small quantities of these chemicals led to mortality. Feeding by nymphs on artificial diets containing synthetic compounds resulted in 100% mortality within 48 h, irrespective of compound. Further testing of ergot alkaloids to characterize the mode of action that leads to psyllid mortality is warranted. |
Databáze: | OpenAIRE |
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