Differentiation between Triatoma arthurneivai and Triatoma wygodzinskyi (Hemiptera: Reduviidae: Triatominae) using cytotaxonomy
| Autor: | Maria Tercília Vilela de Azeredo-Oliveira, Felipe Ferraz Figueiredo Moreira, Kaio Cesar Chaboli Alevi, Carlos Henrique Lima Imperador, José Jurberg |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty 030231 tropical medicine Zoology 03 medical and health sciences 0302 clinical medicine Spermatocytes Genetics medicine Animals Triatoma Molecular Biology Triatominae Phylogeny Cytotaxonomy biology Cytogenetics General Medicine biology.organism_classification Hemiptera Chromatin Chromosomes Insect 030104 developmental biology Reduviidae Triatoma wygodzinskyi Taxonomy (biology) |
| Zdroj: | Genetics and Molecular Research. 15 |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/gmr.15027869 |
| Popis: | Using classic morphometric techniques to examine the head and thorax of Triatoma specimens, researchers identified a possible taxonomic problem involving T. arthurneivai (Lent & Martins) and T. wygodzinskyi (Lent). A recent geometric morphometric study indicated that the insects captured outside the Serra do Cipo region, State of Minas Gerais, Brazil, were T. wygodzinskyi. The misidentification of T. arthurneivai as T. wygodzinskyi could result in several problems associated with entoepidemiological lifting, the biological characterization of the species, and phylogenetic reconstruction. For the first time, we describe the use of cytogenetic analysis as a tool for differentiation between T. arthurneivai and T. wygodzinskyi. The results indicated that both species had the same number of chromosomes 2n = 22 (20A + XY). However, analyses of spermatocytes during early prophase indicated that it was possible to differentiate T. arthurneivai and T. wygodzinskyi, because only T. arthurneivai exhibited heteropycnotic blocks distributed in the chromatin. Therefore, we highlight the analysis of spermatocytes as a taxonomic tool for the characterization of T. arthurneivai and T. wygodzinskyi, and suggest that the technique can be used for entoepidemiological lifting in vector control programs. Thus, the results presented here, in conjunction with morphometric analyses, are of utmost taxonomic and epidemiological importance for the identification of T. arthurneivai and T. wygodzinskyi specimens. |
| Databáze: | OpenAIRE |
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