A cytosolic pathway for MHC class II–restricted antigen processing that is proteasome and TAP dependent
| Autor: | Laurence C. Eisenlohr, Gomathinayagam Sinnathamby, Deepa Rajagopal, Mona K Tewari |
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| Rok vydání: | 2005 |
| Předmět: |
Antigen Presentation
Cytoplasm Proteasome Endopeptidase Complex MHC class II biology CD74 Antigen processing Immunology Antigen presentation Histocompatibility Antigens Class II chemical and pharmacologic phenomena Transporter associated with antigen processing MHC restriction Molecular biology Epitope Mice MHC class I biology.protein Animals Immunology and Allergy ATP-Binding Cassette Transporters Female ATP Binding Cassette Transporter Subfamily B Member 2 Signal Transduction |
| Zdroj: | Nature Immunology. 6:287-294 |
| ISSN: | 1529-2916 1529-2908 |
| Popis: | By convention, presentation of major histocompatibility complex (MHC) class I-restricted epitopes involves processing by cytosolic proteasomes, whereas MHC class II-restricted epitopes are generated by endosomal proteases. Here, we show that two MHC class II-restricted epitopes within influenza virus were generated by a proteasome- and TAP-dependent pathway that was accessed by exogenous virus in dendritic cells (DCs) but not cell types with less permeable endosomes. Both epitopes were presented by recycling MHC class II molecules. Challenging mice with influenza or vaccinia viruses demonstrated that a substantial portion of the MHC class II-restricted response was directed against proteasome-dependent epitopes. By complementing endosomal activities, this pathway broadens the array of MHC class II-restricted epitopes available for CD4(+) T cell activation. |
| Databáze: | OpenAIRE |
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