Regulator DegU is required for multicellular behavior in Lysinibacillus sphaericus
| Autor: | Zhiming Yuan, Yimin Hu, Shen Tian, Quanxin Cai, Xiaomin Hu, Yong Ge |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Bacterial Toxins 030106 microbiology Regulator Swarming motility Motility Bacillus Biology Microbiology 03 medical and health sciences Bacterial Proteins Transcriptional regulation Phosphorylation Molecular Biology Sequence Deletion Spores Bacterial Pore-forming toxin Virulence fungi Biofilm Gene Expression Regulation Bacterial General Medicine Two-component regulatory system Cell biology Phenotype Biofilms Mutation Signal transduction Signal Transduction |
| Zdroj: | Research in Microbiology. 169:177-187 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/j.resmic.2017.12.006 |
| Popis: | DegS and DegU make up a two component system belonging to a class of signal transduction systems that play important roles in a broad range of bacterial responses to the environment. However, little study has been done to explore the physiological functions of DegS-DegU in mosquitocidal Lysinibacillus sphaericus . In this study, it was found that deletion of degU or degS-degU inhibited the swarming motility , biofilm formation, sporulation and binary toxin production through regulating the related genes, and phosphorylation was necessary for the functions of DegU. Based on the findings, a regulation network mediated by DegU was delineated. Both DegU-pi and Spo0A-pi positively regulates genes which are linked with the transition from stage Ⅱ to the end of the sporulation process and also influences the production of binary toxins via regulation on sigE. Both DegU-pi and Spo0A-pi negatively regulate abrB/sinR and influence the biofilm formation. DegU-pi can positively regulate the motility via the regulation on sigD. Whether the regulations are directly or indirectly need to be explored. Moreover, Spo0A-pi may indirectly regulate the swarming motility through negatively regulating DegU. It was concluded that DegU is a global transcriptional regulator on cell swarming motility, biofilm formation, sporulation and virulence in L. sphaericus. |
| Databáze: | OpenAIRE |
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