Prospective value of PCR amplification and sequencing for diagnosis and typing of old world Leishmania infections in an area of nonendemicity
| Autor: | Francine Pratlong, Hung Bui, Jean-Pierre Gangneux, Y. J. F. Garin, Claudine Sarfati, Jean Menotti, Frédéric Lorenzo, Hélène Blanche, Francis Derouin |
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| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Direct examination Molecular Sequence Data Leishmaniasis Cutaneous Polymerase Chain Reaction Sensitivity and Specificity law.invention Species Specificity law Predictive Value of Tests Genotype medicine Parasite hosting Animals Humans Typing Polymerase chain reaction Leishmania biology Base Sequence Kinetoplastida Leishmaniasis Sequence Analysis DNA DNA Protozoan biology.organism_classification medicine.disease Virology Molecular biology Nuclear DNA Culture Media Isoenzymes Leishmaniasis Visceral Parasitology |
| Zdroj: | Journal of clinical microbiology. 41(4) |
| ISSN: | 0095-1137 |
| Popis: | We assessed the prospective value of PCR amplification of a repetitive sequence from Leishmania nuclear DNA and sequencing for the diagnosis and typing of Old World Leishmania infection in an area of nonendemicity. During this 42-month study, 29 of 168 consecutive samples were examined and classified as positive for Leishmania by direct examination and/or in vitro culture. This molecular approach showed excellent sensitivity (97%) and specificity (100%) compared to direct examination (86 and 100%, respectively) and in vitro culture (72 and 100%, respectively). Isoenzymatic and molecular typing allowed similar identification for 12 samples. Besides, PCR and subsequent sequencing of DNA products permitted the species identification of 14 samples for which parasite culture remained negative or did not allow isoenzymatic characterization, indicating the complementarity of parasitological and molecular tools. |
| Databáze: | OpenAIRE |
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