Androgenic regulation of sexually dimorphic expression of RNA binding motif protein 48 in the developing mouse cortex and hippocampus
| Autor: | Allyson Milla, Chris Armoskus, Marilisa Santacruz, Saori Taniguchi, Houng-Wei Tsai, Michael Franklin, Kathy Trang, Courtney Moder |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Testosterone propionate medicine.medical_specialty Hippocampus Estrogen receptor Article Mice 03 medical and health sciences chemistry.chemical_compound Aromatase Sex Factors 0302 clinical medicine Developmental Neuroscience Internal medicine Cortex (anatomy) medicine Animals 030304 developmental biology Cerebral Cortex Sex Characteristics 0303 health sciences Sexual differentiation biology Proteins RNA-Binding Proteins Testosterone Propionate Androgen receptor Endocrinology medicine.anatomical_structure Gene Expression Regulation Receptors Estrogen chemistry Receptors Androgen Androgens biology.protein Female Estrogen receptor alpha 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Int J Dev Neurosci |
| ISSN: | 1873-474X 0736-5748 |
| DOI: | 10.1016/j.ijdevneu.2019.07.011 |
| Popis: | To further reveal the molecular mechanism underlying sexual differentiation of the mouse cerebral cortex and hippocampus, we reanalyzed our previous microarray study with Gene Ontology (GO) term enrichment and found that the GO term “RNA binding” was over-represented among the 89 sexually dimorphic candidate genes. Thus, we selected 16 autosomal genes annotated to the term RNA binding and profiled their mRNA expression in the developing male and female mouse cortex/hippocampus. During the first three weeks after birth, sex differences in mRNA levels of Khdrbs2, Nanos2, Rbm48, and Tdrd3 were observed in the mouse cortex/hippocampus. Of these genes, only the female-biased expression of Rbm48 in neonates was abolished by prenatal exposure to testosterone propionate (TP), while postnatal treatment of TP three weeks after birth increased Rbm48 and Tdrd3 mRNA levels in both sexes. Regardless of sex, the postnatal cortex/hippocampus also showed a marked increase in the content of androgen receptor (Ar) and estrogen receptor β (Esr2), but a decrease in estrogen receptor α (Esr1) and aromatase (Cyp19a1), which might confer the different responses of Rbm48 to prenatal and postnatal TP. Our results suggest that androgen-regulated, sexually dimorphic Rbm48 expression might present a novel molecular mechanism by which perinatal androgens control development of sexual dimorphism in cortical and hippocampal structure and function. |
| Databáze: | OpenAIRE |
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