Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples
| Autor: | Kurbakov, Konstantin A., Konorov, Evgenii A., Minaev, Mikhail Y., Kuznetsova, Oksana A. |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
General Chemical Engineering
lcsh:Biotechnology fermented sausages Industrial and Manufacturing Engineering law.invention Starter law Lactobacillus lcsh:TP248.13-248.65 Multiplex Food science Polymerase chain reaction biology lcsh:TP368-456 starter cultures Lactobacillus sakei food and beverages biology.organism_classification lcsh:Food processing and manufacture Real-time polymerase chain reaction Lactobacillus curvatus Fermentation Preliminary Communications Primer (molecular biology) real-time PCR HRM Food Science Biotechnology |
| Zdroj: | Food Technology and Biotechnology, Vol 57, Iss 1, Pp 97-104 (2019) Food Technology and Biotechnology |
| ISSN: | 1334-2606 1330-9862 |
| Popis: | Optimization of fermentation processes requires monitoring the species composition of starter cultures and their growth during fermentation. Most starter cultures contain closely related species. Nowadays, high-resolution melting (HRM) analysis is extensively used for multiplex identification of closely related species. In the present paper, we applied real-time polymerase chain reaction (PCR) with HRM analysis for the detection and differentiation of Lactobacillus sakei and L. curvatus. A primer pair was selected for the site of the rpoA gene of Lactobacillus spp. Eleven starter cultures and fifteen fermented sausages with a known bacterial composition were successfully tested using real-time PCR with HRM analysis with the developed primer pair. |
| Databáze: | OpenAIRE |
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