Detection of rabbit Haemorrhagic disease virus 2 during the wild rabbit (Oryctolagus cuniculus) eradication from the Berlengas archipelago, Portugal
| Autor: | Carina L. Carvalho, Jorge Correia, Margarida Duarte, F.A. Abade dos Santos, Marta Henriques, Miguel Fevereiro, Maria C. Peleteiro, Oliveira Nuno |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Veterinary medicine Hemorrhagic Disease Virus Rabbit Parasitism Zoology Myxoma virus Oryctolagus cuniculus Virus Rodent Diseases Wild rabbit Rabbit haemorrhagic disease 03 medical and health sciences RHDV2 Cause of Death medicine Animals Berlenga Island Caliciviridae Infections Cause of death lcsh:Veterinary medicine Portugal General Veterinary biology Phylogenetic tree Rabbit haemorrhagic disease virus Berlengas General Medicine biology.organism_classification UNESCO 030104 developmental biology lcsh:SF600-1100 Female Rabbits medicine.symptom Emaciation Research Article |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, Vol 13, Iss 1, Pp 1-10 (2017) |
| ISSN: | 1746-6148 |
| Popis: | Background In the regular wildlife monitoring action carried out in the summer of the past few years at the Berlenga Island, wild rabbits (Oryctolagus cuniculus) have been repeatedly found dead. However, the origin of those deaths was never investigated. Our aim was to investigate the cause of death of 11 rabbits collected between April and May 2016. Results While screening samples from rabbit carcasses for the major viral rabbit pathogens, five tested positive to RHDV2 but all were negative for RHDV and myxoma virus (MYXV). For six RHDV2-negative specimens, emaciation and parasitism were considered the most probable cause of death. Lesions identified in the RHDV2-positive rabbits included non-suppurative diffuse hepatic necrosis and pulmonary lesions varying from congestion and oedema of the lungs to interstitial pneumonia. Sequencing analysis of the vp60 gene obtained from two specimens showed identical vp60 sequences. Comparison with other known RHDV2 strains from public databases through BLAST analysis revealed a closer similarity with strains from Alentejo collected during 2013. Maximum Likelihood and Bayesian phylogenetic analysis showed that the 2016 strains from the archipelago have a higher resemblance with a group of strains mostly collected in the South of Portugal between 2013 and 2014. Conclusion The results suggest that RHDV2 may have been introduced on the Berlenga Island a few years ago, having evolved separately from mainland strains due to insularity. |
| Databáze: | OpenAIRE |
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