Evaluation of the potential protective effects of ad libitum black grape juice against liver oxidative damage in whole-body acute X-irradiated rats
Autor: | Jacqueline da Costa Escobar Piccoli, J.P. Barrio, Javier González-Gallego, V.V.R. Andrade, Paquita González, E.R. Andrade, Ivana Beatrice Mânica da Cruz |
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Rok vydání: | 2011 |
Předmět: |
Male
Antioxidant medicine.medical_treatment Blotting Western DNA Fragmentation Toxicology medicine.disease_cause Thiobarbituric Acid Reactive Substances Beverages Placebos Lipid peroxidation chemistry.chemical_compound medicine TBARS Animals Vitis Food science Rats Wistar chemistry.chemical_classification Glutathione Peroxidase Superoxide Dismutase Chemistry X-Rays Glutathione peroxidase Fructose General Medicine Glutathione Rats Radiography Oxidative Stress Liver Biochemistry DNA fragmentation Lipid Peroxidation Whole-Body Irradiation Oxidative stress Food Science |
Zdroj: | Food and Chemical Toxicology. 49:1026-1032 |
ISSN: | 0278-6915 |
Popis: | Aims The aim of this study was to evaluate the potential protective effects of ad libitum black grape (Vitis labrusca) juice against liver oxidative damage in whole-body acute X-irradiated rats. Main methods Animals were fed ad libitum and drank voluntarily black grape juice or placebo (isocaloric glucose and fructose solution) for 6 days before and 15 days following a 6 Gy X-irradiation from a 200 kV machine. Key findings Irradiated animals receiving placebo showed a significant increase in the concentration of thiobarbituric acid-reactive substances (TBARS), a marker of lipid peroxidation, as well as a significant decrease in both Cu/Zn superoxide dismutase (Cu/ZnSOD) and glutathione peroxidase (GPx) activity and reduced glutathione concentration (GSH). Black grape juice supplementation resulted in a reversal of lipid peroxidation, Cu/ZnSOD activity, and GSH concentration, towards values not significantly differing from those in non-irradiated, placebo-supplemented rats. Poly(ADP-ribose) polymerase (PARP-1) and Cu/ZnSOD changes in protein expression were observed for irradiated rats. No change in p53 expression or DNA fragmentation was found. Significance Ad libitum black grape juice intake is able to restore the liver primary antioxidant system against adverse effects due to whole-body acute X-irradiation in rats after 15 days post-irradiation. The results support using antioxidant supplements as a preventive tool against radiation-induced harm. |
Databáze: | OpenAIRE |
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