Discriminant biomarkers of acute respiratory distress syndrome associated to H1N1 influenza identified by metabolomics HPLC-QTOF-MS/MS platform
| Autor: | José A. Lorente, Andrés Esteban, Laura Righetti, Federica Pellati, Francisco J. Rupérez, J. Gea, Mariano Fernández-López, Alessia Ferrarini, Juan Pablo Horcajada, Antoni J. Betbese, Jordi Ordoñez, Jesús Ruiz Cabello, Ma Paz Martínez, Annalaura Mastrangelo, Nicolás Nin |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine ARDS viruses Metabolite Clinical Biochemistry Pathogenesis Biology Biochemistry Biomarkers Influenza A (H1N1) virus infection Untargeted metabolomics Virus Analytical Chemistry Cohort Studies 03 medical and health sciences chemistry.chemical_compound Influenza A Virus H1N1 Subtype Metabolomics Tandem Mass Spectrometry Influenza Human medicine Metabolome Humans Chromatography High Pressure Liquid Aged Respiratory Distress Syndrome virus diseases Middle Aged medicine.disease Pneumonia 030104 developmental biology chemistry Immunology Female Complication |
| Zdroj: | ELECTROPHORESIS r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau Universitat Pompeu Fabra |
| ISSN: | 0173-0835 |
| DOI: | 10.1002/elps.201700112 |
| Popis: | Acute Respiratory Distress Syndrome (ARDS) is a serious complication of influenza A (H1N1) virus infection. Its pathogenesis is unknown, and biomarkers are lacking. Untargeted metabolomics allows the analysis of the whole metabolome in a biological compartment, identifying patterns associated with specific conditions. We hypothesized that LC-MS could help identify discriminant metabolites able to define the metabolic alterations occurring in patients with influenza A (H1N1) virus infection that developed ARDS. Serum samples from patients diagnosed with 2009 influenza A (H1N1) virus infection with (n = 25) or without (n = 32) ARDS were obtained on the day of hospital admission and analyzed by LC-MS/MS. Metabolite identification was determined by MS/MS analysis and analysis of standards. The specificity of the patterns identified was confirmed in patients without 2009 influenza A(H1N1) virus pneumonia (15 without and 17 with ARDS). Twenty-three candidate biomarkers were found to be significantly different between the two groups, including lysophospholipids and sphingolipids related to inflammation; bile acids, tryptophan metabolites, and thyroxine, related to the metabolism of the gut microflora. Confirmation results demonstrated the specificity of major alterations occurring in ARDS patients with influenza A (H1N1) virus infection. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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