Plasma Cell Proliferation Is Reduced in Myeloma-Induced Hypercalcemia and in Co-Culture with Normal Healthy BM-MSCs
| Autor: | Nader Vazifeh Shiran, Saeid Abroun |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
business.industry
Demineralized bone matrix Biochemistry (medical) Clinical Biochemistry Mesenchymal stem cell Bone Marrow Cells Mesenchymal Stem Cells Plasma cell medicine.disease Coculture Techniques Paracrine signalling medicine.anatomical_structure Apoptosis hemic and lymphatic diseases medicine Cancer research Hypercalcemia Humans Bone marrow Stem cell business Multiple Myeloma Multiple myeloma Cell Proliferation |
| Zdroj: | Laboratory medicine. 52(3) |
| ISSN: | 1943-7730 |
| Popis: | Objective In multiple myeloma (MM), stimulation of osteoclasts and bone marrow (BM) lesions lead to hypercalcemia, renal failure, and anemia. Co-culture of the myeloma cells in both hypocalcemia and hypercalcemia concentrations with bone marrow-mesenchymal stem cells were evaluated. Materials and Methods Viability and survival of myeloma cells were assessed by microculture tetrazolium test and flow cytometric assays. Mesenchymal stem cells (MSCs) were extracted from normal and myeloma patients and were co-cultured with myeloma cells. Results Myeloma cells showed less survival in both hypocalcaemia and hypercalcemia conditions (P Conclusion Normal healthy-MSCs do not protect myeloma cells, but inhibit them. However, increasing the ratio of myeloma cells to MSCs reduces their inhibitory effects of MSCs and leads to their myelomatous transformation. |
| Databáze: | OpenAIRE |
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