Direct thermal desorption gas chromatographic determination of toxicologically relevant concentrations of ethylene glycol in whole blood
| Autor: | Stephen. Townsend, Kevin C. Honeychurch, Paul Bowdler, James. Robson |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Flame Ionization
Ethylene Glycol Chromatography Chromatography Gas 010401 analytical chemistry Extraction (chemistry) Thermal desorption 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry 030220 oncology & carcinogenesis Calibration Electrochemistry Environmental Chemistry Humans Gas chromatography Derivatization Ethylene glycol Spectroscopy Whole blood |
| Zdroj: | The Analyst. 143(4) |
| ISSN: | 1364-5528 |
| Popis: | A simple and rapid method involving thermal desorption gas chromatography (TD-GC) with flame ionisation detection has been successfully developed for the determination of ethylene glycol in whole blood. No sample extraction or derivatization steps were required. The conditions required for the direct determination of ethylene glycol in whole blood were optimised and require only the addition of the internal standard, 1,2-butanediol, to the sample. A 1 μL aliquot of the sample was then introduced to the thermal desorption unit, dried, and thermally desorbed directly to the gas chromatograph. A calibration curve was constructed over the concentration range of 1.0 to 200 mM and was found to be linear over the range investigated with an R |
| Databáze: | OpenAIRE |
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