Simultaneous densitometric determination of anthelmintic drug albendazole and its metabolite albendazole sulfoxide by HPTLC in human plasma and pharmaceutical formulations
| Autor: | Pranav S. Shrivastav, Jui J. Pandya, Mallika Sanyal |
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| Rok vydání: | 2016 |
| Předmět: |
Metabolite
Clinical Biochemistry Pharmaceutical formulation Albendazole 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Drug Stability Limit of Detection Drug Discovery medicine Humans High performance thin layer chromatography Molecular Biology Active metabolite Chromatography High Pressure Liquid Pharmacology Detection limit Anthelmintics Chromatography 010405 organic chemistry 010401 analytical chemistry Reproducibility of Results Sulfoxide General Medicine Repeatability 0104 chemical sciences chemistry Linear Models Chromatography Thin Layer medicine.drug |
| Zdroj: | Biomedical chromatography : BMC. 31(9) |
| ISSN: | 1099-0801 |
| Popis: | A new, simple, accurate and precise high-performance thin-layer chromatographic method has been developed and validated for simultaneous determination of an anthelmintic drug, albendazole, and its active metabolite albendazole, sulfoxide. Planar chromatographic separation was performed on aluminum-backed layer of silica gel 60G F254 using a mixture of toluene-acetonitrile-glacial acetic acid (7.0:2.9:0.1, v/v/v) as the mobile phase. For quantitation, the separated spots were scanned densitometrically at 225 nm. The retention factors (Rf ) obtained under the established conditions were 0.76 ± 0.01 and 0.50 ± 0.01 and the regression plots were linear (r2 ≥ 0.9997) in the concentration ranges 50-350 and 100-700 ng/band for albendazole and albendazole sulfoxide, respectively. The method was validated for linearity, specificity, accuracy (recovery) and precision, repeatability, stability and robustness. The limit of detection and limit of quantitation found were 9.84 and 29.81 ng/band for albendazole and 21.60 and 65.45 ng/band for albendazole sulfoxide, respectively. For plasma samples, solid-phase extraction of analytes yielded mean extraction recoveries of 87.59 and 87.13% for albendazole and albendazole sulfoxide, respectively. The method was successfully applied for the analysis of albendazole in pharmaceutical formulations with accuracy ≥99.32%. |
| Databáze: | OpenAIRE |
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