A potent activator of HIV-1 replication is present in the genital tract of a subset of HIV-1-infected and uninfected women
| Autor: | Nancy Glick, Constance A. Benson, Beverly E. Sha, M N Saarloos, Mary K. Hayden, G T Spear, Kenneth A. Roebuck, Alan L. Landay, Lena Al-Harthi, L S Massad |
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| Rok vydání: | 1997 |
| Předmět: |
Sexually transmitted disease
Transcription Genetic T-Lymphocytes Immunology HIV Core Protein p24 HIV Infections Biology Peripheral blood mononuclear cell Virus Monocytes Heating HIV Seronegativity Virus latency Endopeptidases HIV Seropositivity medicine Immunology and Allergy Humans Primary isolate Therapeutic Irrigation Cells Cultured HIV Long Terminal Repeat Tumor Necrosis Factor-alpha Genitalia Female medicine.disease Virology Infectious Disease Transmission Vertical Virus Latency Infectious Diseases HIV-1 Female Viral disease |
| Zdroj: | AIDS (London, England). 11(11) |
| ISSN: | 0269-9370 |
| Popis: | Objective and design To determine whether the female genital tract contains factors that affect HIV-1 replication. Cervicovaginal lavage (CVL) samples were collected from HIV-1-seropositive and seronegative women and added to cell cultures. Methods HIV p24 production was used to measure the effects of CVL on replication of HIVMN in a T-cell line, of a primary isolate in peripheral blood mononuclear cells, or on HIV expression by the latently-infected monocytic U1 cell line. The effects of CVL on the HIV long terminal repeat (LTR) were determined in 1G5 T cells by measuring luciferase activity. Results Increased replication of HIVMN and a primary isolate were observed in T cells cultured with CVL samples from three out of 38 HIV-infected women, one out of four uninfected high-risk women, and none of 12 low-risk women. The CVL factor increased replication by enhancing virus expression via activation of the HIV LTR. The HIV-inducing activity was highly stable to heat but was sensitive to proteases, indicating that the activity was distinct from heat-labile cytokines including tumour necrosis factor-alpha. Conclusions This is the first study to show that a factor which can stimulate HIV-1 replication is present at biologically active levels in the reproductive tract of women. This factor could potentially affect sexual or vertical transmission of HIV-1 by altering genital tract virus load or virus expression. |
| Databáze: | OpenAIRE |
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