Excretion of amino acids in childhood. A reliable method for the determination of amino acid nitrogen in urine
| Autor: | C. Danelatou-Athanassiadou, N.S. Constantsas |
|---|---|
| Rok vydání: | 1964 |
| Předmět: |
Adolescent
Fever Urease Nitrogen Physiology Coefficient of variation Clinical Biochemistry Inorganic chemistry chemistry.chemical_element Urine Kidney Biochemistry Excretion chemistry.chemical_compound Ammonia Urea Amino Acids Renal Aminoacidurias Child Amino Acid Metabolism Inborn Errors chemistry.chemical_classification Chromatography biology Biochemistry (medical) Collagen Diseases General Medicine Creatine Body Fluids Amino acid chemistry Creatinine biology.protein Colorimetry Indicators and Reagents |
| Zdroj: | Clinica Chimica Acta. 9:1-12 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(64)90038-5 |
| Popis: | A reliable and relatively simple colorimetric determination of amino acid nitrogen in urine is described; urea and ammonia are completely eliminated. The average coefficient of variation calculated from 39 triplicate analyses is ± 3.7%. Colour yields of the main free amino acids occurring in the urine of children are established in relation to the most satisfactory composition of a standard for the determination. Equilibration of urine with ion exchangers for the selective elimination of ammonia from urine is experimentally evaluated. Data for the computation of approximate urea concentration from the pH of urease-buffer treated urine, are given. Averages and standard deviations are given for the ratio of amino acid nitrogen to creatinine nitrogen at different ages in childhood. Detailed statistical analyses are presented which provide conclusive evidence for the enhancement of amino-aciduria with decreasing age, as well as in cases of fever, and collagen diseases. |
| Databáze: | OpenAIRE |
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