Chaetomium globosum extract mediated gold nanoparticle synthesis and potent anti-inflammatory activity
| Autor: | Kyathegowdanadoddi Srinivasa Balaji, Uzma Munawer, Sunayana Ningaraju, Vinay B. Raghavendra, Arivalagan Pugazhendhi, Kathirvel Brindhadevi, Govindappa Melappa |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Xanthine Oxidase Vitex negundo Lipoxygenase Anti-Inflammatory Agents Biophysics Metal Nanoparticles Infrared spectroscopy Chaetomium Carrageenan 01 natural sciences Biochemistry Catechin Vitex HeLa Mice 03 medical and health sciences chemistry.chemical_compound Endophytes Animals Edema Humans Fourier transform infrared spectroscopy Cytotoxicity Molecular Biology Acetic Acid 030304 developmental biology Inflammation 0303 health sciences Behavior Animal biology Chaetomium globosum 010401 analytical chemistry Cell Biology Hydrogen-Ion Concentration biology.organism_classification 0104 chemical sciences Plant Leaves Disease Models Animal chemistry Cyclooxygenase 2 Colloidal gold Female Gold HeLa Cells Nuclear chemistry |
| Zdroj: | Analytical Biochemistry. 612:113970 |
| ISSN: | 0003-2697 |
| Popis: | Gold nanoparticles (AuNPs) are gaining a lot of attention in recent decades from researchers due to their unique optoelectronic properties and their significance in the field of biomedicine. Keeping this in view, our research work was designed to investigate gold nanoparticles obtained by using a fungal endophytic strain Chaetomium globosum, isolated from Vitex negundo which showed significant activity on enzyme inhibition. In the present study, the fungal isolate C. globosum was characterized using HPLC and LC-MS. A novel compound Catechin was matched with standard Catechin. Further, the endophyte C. globosum extract was utilized to synthesize gold nanoparticles (CgAuNPs) which was analysed by UV–visible spectroscopy. The CgAuNPs exhibited wine red color and the absorption peak appeared at 542 nm confirming the formation of the AuNPs. Further, Fourier Transmission Infrared Spectroscopy (FTIR) was performed to confirm the various functional groups present in mycosynthesized CgAuNPs. FTIR analysis demonstrated the presence of amines, flavonoids, as well as the presence of amide I linkage which possibly reduces Au+ to Au0. The synthesized CgAuNPs exhibited potential cytotoxicity against HeLa cells in a dose dependent manner. Further, CgAuNPs demonstrated significant anti-inflammatory activity. Overall, the present work provides insights into the design of nano delivery and may be applied for clinical studies in future. |
| Databáze: | OpenAIRE |
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