Identification of a Family of Fatty-Acid-Speciated Sonic Hedgehog Proteins, Whose Members Display Differential Biological Properties
| Autor: | Neal S. Schilling, Anthony J. Capobianco, Stephane Houel, William M. Old, Samer Singh, Natalie G. Ahn, Jun Long, Robert Tokhunts, Jezabel Rodgriguez-Blanco, David J. Robbins |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
animal structures
Chick Embryo Article General Biochemistry Genetics and Molecular Biology Cell Line 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Animals Humans Hedgehog Proteins Sonic hedgehog Hedgehog Lipid raft lcsh:QH301-705.5 Chromatography High Pressure Liquid 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Fatty Acids Fatty acid Biological activity Metabolism Biochemistry chemistry lcsh:Biology (General) Cell culture embryonic structures biology.protein Peptides Chickens 030217 neurology & neurosurgery Biogenesis |
| Zdroj: | Cell Reports, Vol 10, Iss 8, Pp 1280-1287 (2015) |
| ISSN: | 2211-1247 |
| Popis: | SummaryHedgehog (HH) proteins are proteolytically processed into a biologically active form that is covalently modified by cholesterol and palmitate. However, most studies of HH biogenesis have characterized protein from cells in which HH is overexpressed. We purified Sonic Hedgehog (SHH) from cells expressing physiologically relevant levels and showed that it was more potent than SHH isolated from overexpressing cells. Furthermore, the SHH in our preparations was modified with a diverse spectrum of fatty acids on its amino termini, and this spectrum of fatty acids varied dramatically depending on the growth conditions of the cells. The fatty acid composition of SHH affected its trafficking to lipid rafts as well as its potency. Our results suggest that HH proteins exist as a family of diverse lipid-speciated proteins that might be altered in different physiological and pathological contexts in order to regulate distinct properties of HH proteins. |
| Databáze: | OpenAIRE |
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