High-performance liquid chromatography assay of N,N-dimethyl-p-toluidine released from bone cements: evidence for toxicity
| Autor: | C. Zolezzi, S. Stea, D. Cavedagna, Gabriela Ciapetti, M. Visentin, Donatella Granchi, Arturo Pizzoferrato |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Materials science Toluidines Cell Survival Biophysics Bioengineering Bone Neoplasms High-performance liquid chromatography Biomaterials medicine Tumor Cells Cultured Humans Dimethyl-p-toluidine Curing (chemistry) Chromatography High Pressure Liquid Osteosarcoma Aqueous solution Chromatography Osteoblasts Cell Cycle Bone Cements Bone cement In vitro Surgery Kinetics Mechanics of Materials Toxicity Ceramics and Composites Amine gas treating |
| Zdroj: | Biomaterials. 18(3) |
| ISSN: | 0142-9612 |
| Popis: | Five commercially available bone cements were analysed by high-performance liquid chromatography for detecting the residual content of an accelerator, the amine N,N-dimethyl-p-toluidine (DMPT), after curing. It was found that the concentration of DMPT in aqueous extracts decreases with time, being almost absent 7 days after curing. Differences were noticed among the cements; residual DMPT is higher in cements prepared with higher content of the amine. It is verified that DMPT's toxic effect on cell cultures is dose-related; a delay in the cell replication cycle is induced in vitro. Damage is reversible, thus justifying the low bone cement toxicity that is clinically ascertained. |
| Databáze: | OpenAIRE |
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