Multiplex cell microarrays for high-throughput screening
| Autor: | Sinan K. Muldur, Ophélie I. Berthuy, François Rossi, Loïc J. Blum, Pascal Colpo, Christophe A. Marquette |
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| Přispěvatelé: | Génie Enzymatique, Membrane Biomimétique et Assemblages Supramoléculaires (GEMBAS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), JRC Institute for Health and Consumer Protection (IHCP), European Commission - Joint Research Centre [Ispra] (JRC) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microarray High-throughput screening Cell Biomedical Engineering Bioengineering Nanotechnology 02 engineering and technology Biology Biochemistry 03 medical and health sciences medicine Animals Humans Multiplex [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] [CHIM.ORGA]Chemical Sciences/Organic chemistry Drug discovery General Chemistry 021001 nanoscience & nanotechnology Flow Cytometry High-Throughput Screening Assays 030104 developmental biology medicine.anatomical_structure Tissue Array Analysis Protein microarray Gene chip analysis DNA microarray Single-Cell Analysis 0210 nano-technology |
| Zdroj: | Lab on a Chip Lab on a Chip, Royal Society of Chemistry, 2016, 16, pp.4248-4262. ⟨10.1039/C6LC00831C⟩ |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/C6LC00831C⟩ |
| Popis: | International audience; Microarray technology was developed in the early 1990s to measure the transcription levels of thousands of genes in parallel. The basic premise of high-density arraying has since been expanded to create cell microarrays. Cells on chip are powerful experimental tools for high-throughput and multiplex screening of samples or cellular functions. Miniaturization increases assay throughput while reducing both reagent consumption and cell population heterogeneity effect, making these systems attractive for a wide range of assays, from drug discovery to toxicology, stem cell research and therapy. It is usual to functionalize the surface of a substrate to design cell microarrays. One form of cell microarrays, the transfected cell microarray, wherein plasmid DNA or siRNA spotted on the surface of a substrate is reverse-transfected locally into adherent cells, has become a standard tool for parallel cell-based analysis. With the advent of technology, cells can also be directly spotted onto functionalized surfaces using robotic fluid-dispensing devices or printed directly on bio-ink material. We are providing herein an overview of the latest developments in optical cell microarrays allowing high-throughput and high-content analysis. |
| Databáze: | OpenAIRE |
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