Inhibition of Leishmania (Leishmania) amazonensis growth and infectivity by aureobasidin A
| Autor: | Anita H. Straus, Ameria K. Tanaka, Valderez Bastos Valero, Helio K. Takahashi |
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| Rok vydání: | 2007 |
| Předmět: |
Pharmacology
Microbiology (medical) Infectivity Mice Inbred BALB C biology Dose-Response Relationship Drug Inoculation Macrophages Leishmania mexicana Kinetoplastida biology.organism_classification Leishmania Glycosphingolipids Microbiology Mice Infectious Diseases Depsipeptides parasitic diseases Protozoa Animals Pharmacology (medical) MTT assay Enzyme Inhibitors Amastigote IC50 |
| Zdroj: | The Journal of antimicrobial chemotherapy. 59(3) |
| ISSN: | 0305-7453 |
| Popis: | To study the effect of aureobasidin A, an inhibitor of inositol phosphorylceramide (IPC) synthase, on Leishmania growth and infectivity.Effects of aureobasidin A were determined for: (i) promastigote growth in axenic culture; (ii) promastigote infectivity in macrophage monolayers; (iii) development of footpad lesions in BALB/c mice; (iv) differentiation of amastigotes into promastigotes.Aureobasidin A (20 microM) inhibited 90% of Leishmania (Leishmania) amazonensis promastigote growth in axenic culture, but the parasites remained viable, i.e. growth curves returned to normal after aureobasidin A was removed from culture medium. The aureobasidin A IC50 was determined by MTT assay as 4.1 microM for L. (L.) amazonensis promastigotes, 12.6 microM for Leishmania (Leishmania) major and 13.7 microM for Leishmania (Viannia) braziliensis. There was a significant delay in infection when L. (L.) amazonensis promastigotes pre-treated with aureobasidin A were inoculated into BALB/c mouse footpads. When aureobasidin A was added to cultured macrophages infected with amastigotes, the number of infected macrophages was reduced by90%.Aureobasidin A is an interesting pharmacological tool to investigate the effect of lipid metabolism inhibition in Leishmania spp. |
| Databáze: | OpenAIRE |
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