Methylopila helvetica sp. nov. and Methylobacterium dichloromethanicum sp. nov. — Novel Aerobic Facultatively Methylotrophic Bacteria Utilizing Dichloromethane
| Autor: | Boris B. Kuznetsov, Yuri A. Trotsenko, Tatjana P. Tourova, Thomas Leisinger, Nina V. Doronina |
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| Rok vydání: | 2000 |
| Předmět: |
Methylene Chloride
Gram-Negative Aerobic Bacteria biology Sequence analysis Molecular Sequence Data Ribosomal RNA biology.organism_classification 16S ribosomal RNA DNA Ribosomal Applied Microbiology and Biotechnology Microbiology Bacterial Typing Techniques Biodegradation Environmental Methylobacterium Chemotaxonomy RNA Ribosomal 16S Methylopila helvetica Energy source Phylogeny Ecology Evolution Behavior and Systematics Bacteria |
| Zdroj: | Systematic and Applied Microbiology. 23:210-218 |
| ISSN: | 0723-2020 |
| DOI: | 10.1016/s0723-2020(00)80007-7 |
| Popis: | Eight strains of Gram-negative, aerobic, asporogenous, neutrophilic, mesophilic, facultatively methylotrophic bacteria are taxonomically described. These icl- serine pathway methylobacteria utilize dichloromethane, methanol and methylamine as well as a variety of polycarbon compounds as the carbon and energy source. The major cellular fatty acids of the non-pigmented strains DM1, DM3, and DM5 to DM9 are C18:1, C16:0, C18:0, Ccy19:0 and that of the pink-pigmented strain DM4 is C18:1. The main quinone of all the strains is Q-10. The non-pigmented strains have similar phenotypic properties and a high level of DNA-DNA relatedness (81-98%) as determined by hybridization. All strains belong to the alpha-subgroup of the alpha-Proteobacteria. 16S rDNA sequence analysis led to the classification of these dichloromethane-utilizers in the genus Methylopila as a new species - Methylopila helvetica sp.nov. with the type strain DM9 (=VKM B-2189). The pink-pigmented strain DM4 belongs to the genus Methylobacterium but differs from the known members of this genus by some phenotypic properties, DNA-DNA relatedness (14-57%) and 16S rDNA sequence. Strain DM4 is named Methylobacterium dichloromethanicum sp. nov. (VKM B-2191 = DSMZ 6343). |
| Databáze: | OpenAIRE |
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