Molecular targeting of bioconjugated graphene oxide nanocarriers revealed at a cellular level using label-free Raman imaging
| Autor: | Adriana Annušová, Silvia Pastorekova, Peter Siffalovic, Eva Majková, Nikola Bugarova, Michal Bodík, Ivana Kajanova, Martina Labudova, Miriam Zaťovičová, Mária Omastová |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Confocal
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Microscopy Atomic Force Spectrum Analysis Raman Endocytosis Cell Line 03 medical and health sciences Dogs Carbonic anhydrase Animals General Materials Science Molecular Targeted Therapy 030304 developmental biology Drug Carriers 0303 health sciences Microscopy Confocal Bioconjugation biology Chemistry Cell sorting Flow Cytometry 021001 nanoscience & nanotechnology Transmembrane protein Biotinylation biology.protein Biophysics Nanoparticles Molecular Medicine Graphite Nanocarriers 0210 nano-technology |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 30:102280 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2020.102280 |
| Popis: | Two-dimensional materials as graphene oxide (GO) are able to accommodate labels as well as toxins for diagnostics and therapy, respectively. The transmembrane protein carbonic anhydrase (CA IX) is one of the molecules selectively expressed by tumor cells. Here, we demonstrate bioconjugation of GO to biotinylated M75 antibody highly selective towards CA IX. Based on a model system, binding between the bioconjugated GO-M75 and Madin-Darby Canine Kidney (MDCK) cells was evaluated. As proven by fluorescence-activated cell sorting, higher intake was observed for GO-M75 towards MDCK cells ectopically expressing CA IX protein on their surface when compared to control MDCK. In particular, we were able to localize GO nanocarrier crossing the membrane during endocytosis, thanks to the optical cross-sectioning of living cells in real-time employed the label-free confocal Raman microscopy. The increased affinity of the prepared GO-M75 molecular complexes validates the use of two-dimensional materials for future strategies of targeted cancer treatment. |
| Databáze: | OpenAIRE |
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