Conserved sequences of BART and BHRF regions encoding viral microRNAs in Epstein-Barr virus-associated lymphoma
| Autor: | Tsunekazu Hishima, Harutaka Katano, Keishin Sunagawa, Hitomi Fukumoto, Hideki Hasegawa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Epstein-Barr Virus Infections Herpesvirus 4 Human Lymphoma lcsh:Medicine Biology medicine.disease_cause Genome Polymerase Chain Reaction General Biochemistry Genetics and Molecular Biology DNA sequencing Virus Conserved sequence 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine hemic and lymphatic diseases Sequence medicine Humans Epstein-Barr virus lcsh:Science (General) lcsh:QH301-705.5 Conserved Sequence miRNA Genetics Mutation Base Sequence lcsh:R General Medicine medicine.disease Virology Epstein–Barr virus MicroRNAs Research Note 030104 developmental biology PCR lcsh:Biology (General) chemistry 030220 oncology & carcinogenesis RNA Viral DNA lcsh:Q1-390 |
| Zdroj: | BMC Research Notes BMC Research Notes, Vol 10, Iss 1, Pp 1-5 (2017) |
| ISSN: | 1756-0500 |
| Popis: | Objective Epstein-Barr virus (EBV) encodes at least 25 pri-microRNAs (miRNAs) in two regions of its DNA genome, BART and BHRF. B95-8, an EBV reference strain, has a deletion in the BART region. However, no information is available on the deletions or mutations in the BART and BHRF regions in clinical samples of EBV-associated lymphoma. Results Nine DNA fragments encoding miR-BARTs and two coding miR-BHRF1s were amplified by PCR from DNA samples extracted from 16 cases of EBV-associated lymphoma. All the PCR products were sequenced directly. DNA fragments encoding miR-BARTs and miR-BHRF1-1 were successfully amplified from all samples. An adenine-to-guanine mutation in the DNA fragment encoding miR-BART2-3p was detected in four of the 16 cases, and a cytosine-to-thymidine mutation in the DNA fragment encoding miR-BART11-3p was detected in one of the 16 samples. These mutations were not associated with any histological categories of lymphoma. In conclusion, mutations were rarely observed in the DNA encoding viral miRNAs in cases of lymphoma. This suggests that the DNA sequences of EBV-encoded miR-BARTs and miR-BHRF1-1 are conserved in EBV-associated lymphoma. |
| Databáze: | OpenAIRE |
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