Influence of Cellular Composition and Exogenous Activation on Growth Factor and Cytokine Concentrations in Canine Platelet-Rich Plasmas
| Autor: | Samuel P. Franklin, Benjamin M. Brainard, Bridget C. Garner, Alena Strelchik, Kate E. Birdwhistell |
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| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
040301 veterinary sciences medicine.medical_treatment canine Fibrinogen 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine growth factors medicine platelet activation Platelet Platelet activation Original Research 030222 orthopedics General Veterinary Growth factor platelet-rich plasma 04 agricultural and veterinary sciences cytokines Vascular endothelial growth factor Endocrinology Cytokine chemistry Platelet-rich plasma Veterinary Science medicine.drug Transforming growth factor |
| Zdroj: | Frontiers in Veterinary Science |
| ISSN: | 2297-1769 |
| Popis: | Objective The purposes of this study were to (1) evaluate correlations among platelet, leukocyte, growth factor, and cytokine concentrations in canine platelet-rich plasmas (PRPs) produced from five different canine PRP-concentrating systems and (2) compare the effects of different activation protocols on platelet activation and growth factor release from one of these PRPs. Methods PRP was made using blood from 15 dogs and each of 5 different PRP systems in a cross-over design. Complete blood counts were performed to quantify platelet and leukocyte concentrations. PRPs were activated, or not, according to manufacturer instructions, and transforming growth factor-β1 (TGF-β1), platelet-derived growth factor-BB (PDGF-BB), vascular endothelial growth factor, and tumor necrosis factor-alpha (TNF-α) were quantified. Differences among platelet, leukocyte, and growth factor concentration were compared among the different systems. Correlations between platelet and anabolic growth factor concentrations were assessed. Subsequently, PRP was made from 12 additional dogs using one of the devices. Each PRP was divided into three aliquots that were activated with calcium chloride (CaCl2), human γ-thrombin (HGT), or not activated. Expression of CD62P and platelet-bound fibrinogen (CAP1) was quantified for each activation group. Concentrations of TGF-β1, PDGF-BB, and TNF-α were also quantified for each activation group and a fourth group that was frozen/thawed. Differences among activation groups were assessed by a Friedman test. Results There were statistically significant differences among the PRPs made with difference devices with regard to platelet, leukocyte, TGF-β1, and PDGF-BB concentrations (p |
| Databáze: | OpenAIRE |
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